Key features and details
- Rabbit polyclonal to beta IV Tubulin
- Suitable for: ICC/IF, IP, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-beta IV Tubulin antibody
See all beta IV Tubulin primary antibodies
DescriptionRabbit polyclonal to beta IV Tubulin
Tested applicationsSuitable for: ICC/IF, IP, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Cynomolgus monkey
Recombinant full length protein corresponding to Human beta IV Tubulin aa 1-444.
MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVY YNEATGGNYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNN WAKGHYTEGAELVDAVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTL LISKIREEFPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETY CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADL RKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMM AACDPRHGRYLTVAAVFRGRMSMKEVDEQMLSVQSKNSSYFVEWIPNNVK TAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTG EGMDEMEFTEAESNMNDLVSEYQQYQDATAEEGEFEEEAEEEVA
Database link: P04350
- WB: 293T and HeLa whole cell lysates. IP: HeLa cell whole lysate. ICC/IF: MCF7 cells. IHC-P: Human ovarian cancer tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab222822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IP||1/200 - 1/2000.|
|WB||1/500 - 1/5000. Detects a band of approximately 50, 58 kDa (predicted molecular weight: 50 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Sequence similaritiesBelongs to the tubulin family.
DomainThe highly acidic C-terminal region may bind cations such as calcium.
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Beta 4 antibody
- Beta 4 tubulin antibody
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All lanes : Anti-beta IV Tubulin antibody (ab222822) at 1/500 dilution
Lane 1 : 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Predicted band size: 50 kDa
Observed band size: 50,58 kDa why is the actual band size different from the predicted?
MCF7 (human breast adenocarcinoma cell line) cells stained for beta IV Tubulin (green) using ab222822 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Paraffin-embedded human ovarian cancer tissue stained for beta IV Tubulin using ab222822 at 1/100 dilution in immunohistochemical analysis.
beta IV Tubulin was immunoprecipitated from 0.5 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab222822 at 1/200 dilution. Western blot was performed from the immunoprecipitate using ab222822 at 1/500 dilution.
Lane 1: Rabbit monoclonal IgG (1 µg) instead of ab222822 in Hela whole cell lysate.
Lane 2: ab222822 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate (10 μg).
ab222822 has not yet been referenced specifically in any publications.