Recombinant
RabMAb

Recombinant Anti-beta IV Tubulin antibody [EPR16775] (ab179504)

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Overview

  • Product name

    Anti-beta IV Tubulin antibody [EPR16775]
    See all beta IV Tubulin primary antibodies

  • Description

    Rabbit monoclonal [EPR16775] to beta IV Tubulin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human beta IV Tubulin aa 50-150. The exact sequence is proprietary.
    Database link: P04350

  • Positive control

    • WB: Human fetal brain, Human cerebellum and Human fetal kidney lysates; HeLa, Jurkat, Neuro-2a, C6, RAW 264.7, and PC-12 whole cell lysates; Mouse brain, heart, kidney and spleen lysates; Rat brain and heart lysates. IHC-P: Human cerebral cortex, Mouse hippocampus and Rat thalamus tissues. ICC/IF: HeLa cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab179504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ICC/IF 1/100.
Flow Cyt 1/20.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

  • Sequence similarities

    Belongs to the tubulin family.

  • Domain

    The highly acidic C-terminal region may bind cations such as calcium.

  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.

  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt

  • Database links

    • Alternative names

      • Beta 4 antibody
      • Beta 4 tubulin antibody
      • beta 5 antibody
      • beta four tubulin antibody
      • Dystonia 4 torsion (autosomal dominant) antibody
      • MC1R antibody
      • TBB4_HUMAN antibody
      • TUB B4 antibody
      • TUBB 4 antibody
      • tubb4 antibody
      • TUBB4A antibody
      • TUBB5 antibody
      • Tubulin 5 beta antibody
      • Tubulin beta 3 antibody
      • Tubulin beta 4 antibody
      • Tubulin beta 4 chain antibody
      • Tubulin beta 4A class IVa antibody
      • Tubulin beta 5 antibody
      • Tubulin beta IV antibody
      • Tubulin beta-4 chain antibody
      see all

    Images

    • Western blot - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)
      Western blot - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)
      All lanes : Anti-beta IV Tubulin antibody [EPR16775] (ab179504) at 1/5000 dilution

      Lane 1 : Human fetal brain lysates
      Lane 2 : Human cerebellum lysates
      Lane 3 : Human fetal kidney lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

      Predicted band size: 50 kDa
      Observed band size: 50 kDa



      Blocking/dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)
      Western blot - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)
      All lanes : Anti-beta IV Tubulin antibody [EPR16775] (ab179504) at 1/5000 dilution

      Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
      Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
      Lane 3 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

      Predicted band size: 50 kDa
      Observed band size: 50 kDa



      Blocking/dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)
      Western blot - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)
      All lanes : Anti-beta IV Tubulin antibody [EPR16775] (ab179504) at 1/5000 dilution

      Lane 1 : Mouse brain lysates
      Lane 2 : Mouse heart lysates
      Lane 3 : Mouse kidney lysates
      Lane 4 : Mouse spleen lysates
      Lane 5 : Rat brain lysates
      Lane 6 : Rat heart lysates
      Lane 7 : Rat kidney lysates
      Lane 8 : Rat spleen lysates
      Lane 9 : C6 (Rat glial tumor cells) whole cell lysates
      Lane 10 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
      Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
      Lane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

      Predicted band size: 50 kDa
      Observed band size: 50 kDa



      Blocking/dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)
      Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling beta IV Tubulin with ab179504 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody (ab150077) at 1/200 dilution (green). Cytoplasm staining is observed.

      Tubulin is detected with Mouse anti-Tubulin antibody (ab7291) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/400 dilution (red). The nuclear counter stain is DAPI (blue).

       

      The  negative controls:-

      -ve control 1 - ab179504 at 1/100 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/400 dilution.
      -ve control 2 -  Mouse anti-Tubulin antibody (ab7291) at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)

      Immunohistochemical analysis of paraffin-embedded Human cerebral cortex issue labeling beta IV Tubulin with ab179504 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Cytoplasm staining is observed on neurons of cerebral cortex.

      Negative control used PBS instead of ab179504, followed by prediluted HRP Polymer for Rabbit/Mouse IgG.

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)

      Immunohistochemical analysis of paraffin embedded Mouse hippocampus tissue labeling beta IV Tubulin with ab179504 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Cytoplasm staining is observed on neurons of mouse hippocampus.

      Negative control used PBS instead of ab179504, followed by prediluted HRP Polymer for Rabbit/Mouse IgG.

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)

      Immunohistochemical analysis of paraffin-embedded Rat thalamus tissue labeling beta IV Tubulin with ab179504 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Cytoplasm staining is observed on neurons of rat thalamus.

      Negative control used PBS instead of ab179504, followed by prediluted HRP Polymer for Rabbit/Mouse IgG.

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)
      Flow Cytometry - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)

      Flow cytometry analysis of 2% paraformaldehyde fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling beta IV Tubulin with ab179504 at 1/20 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is rabbit monoclonal IgG (black line).

    References

    This product has been referenced in:

    • Bi B  et al. Quantitative Proteomic Analysis Reveals Impaired Axonal Guidance Signaling in Human Postmortem Brain Tissues of Chronic Traumatic Encephalopathy. Exp Neurobiol 28:362-375 (2019). Read more (PubMed: 31308796) »
    • Royston L  et al. Viral chimeras decrypt the role of enterovirus capsid proteins in viral tropism, acid sensitivity and optimal growth temperature. PLoS Pathog 14:e1006962 (2018). Read more (PubMed: 29630666) »
    See all 3 Publications for this product

    Publishing research using ab179504? Please let us know so that we can cite the reference in this datasheet.

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