Recombinant Anti-beta IV Tubulin antibody [EPR16775] (ab179504)
![Western blot - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)](https://www.abcam.com/ps/products/179/ab179504/Images/ab179504-228595-anti-beta-iv-tubulin-antibody-epr16775-western-blot.jpg "Western blot - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16775] to beta IV Tubulin
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-beta IV Tubulin antibody [EPR16775]
See all beta IV Tubulin primary antibodies -
Description
Rabbit monoclonal [EPR16775] to beta IV Tubulin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal brain, Human cerebellum and Human fetal kidney lysates; HeLa, Jurkat, Neuro-2a, C6, RAW 264.7, and PC-12 whole cell lysates; Mouse brain, heart, kidney and spleen lysates; Rat brain and heart lysates. IHC-P: Human cerebral cortex, Mouse hippocampus and Rat thalamus tissues. ICC/IF: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16775 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab179504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| WB |
1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
|
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| ICC/IF |
1/100.
|
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| Flow Cyt (Intra) |
1/20.
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| Notes |
|---|
|
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
|
ICC/IF
1/100. |
|
Flow Cyt (Intra)
1/20. |
Target
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Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. -
Sequence similarities
Belongs to the tubulin family. -
Domain
The highly acidic C-terminal region may bind cations such as calcium. -
Post-translational
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 10382 Human
- Entrez Gene: 22153 Mouse
- Entrez Gene: 29213 Rat
- Omim: 602662 Human
- SwissProt: P04350 Human
- SwissProt: Q9D6F9 Mouse
- Unigene: 110837 Human
- Unigene: 7420 Mouse
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Alternative names
- Beta 4 antibody
- Beta 4 tubulin antibody
- beta 5 antibody
see all
Images
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Western blot - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)All lanes : Anti-beta IV Tubulin antibody [EPR16775] (ab179504) at 1/5000 dilution
Lane 1 : Human fetal brain lysates
Lane 2 : Human cerebellum lysates
Lane 3 : Human fetal kidney lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDaBlocking/dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)](https://www.abcam.com/ps/products/179/ab179504/Images/ab179504-228612-anti-beta-iv-tubulin-antibody-epr16775-western-blot.jpg)
Western blot - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)All lanes : Anti-beta IV Tubulin antibody [EPR16775] (ab179504) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
Lane 3 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDaBlocking/dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)](https://www.abcam.com/ps/products/179/ab179504/Images/ab179504-228613-anti-beta-iv-tubulin-antibody-epr16775-western-blot.jpg)
Western blot - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)All lanes : Anti-beta IV Tubulin antibody [EPR16775] (ab179504) at 1/5000 dilution
Lane 1 : Mouse brain lysates
Lane 2 : Mouse heart lysates
Lane 3 : Mouse kidney lysates
Lane 4 : Mouse spleen lysates
Lane 5 : Rat brain lysates
Lane 6 : Rat heart lysates
Lane 7 : Rat kidney lysates
Lane 8 : Rat spleen lysates
Lane 9 : C6 (Rat glial tumor cells) whole cell lysates
Lane 10 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDaBlocking/dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)](https://www.abcam.com/ps/products/179/ab179504/Images/ab179504-228641-anti-beta-iv-tubulin-antibody-epr16775-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling beta IV Tubulin with ab179504 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody (ab150077) at 1/200 dilution (green). Cytoplasm staining is observed.
Tubulin is detected with Mouse anti-Tubulin antibody (ab7291) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/400 dilution (red). The nuclear counter stain is DAPI (blue).
The negative controls:-
-ve control 1 - ab179504 at 1/100 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/400 dilution.
-ve control 2 - Mouse anti-Tubulin antibody (ab7291) at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)](https://www.abcam.com/ps/products/179/ab179504/Images/ab179504-228632-anti-beta-iv-tubulin-antibody-epr16775-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)Immunohistochemical analysis of paraffin-embedded Human cerebral cortex issue labeling beta IV Tubulin with ab179504 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Cytoplasm staining is observed on neurons of cerebral cortex.
Negative control used PBS instead of ab179504, followed by prediluted HRP Polymer for Rabbit/Mouse IgG.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)](https://www.abcam.com/ps/products/179/ab179504/Images/ab179504-228635-anti-beta-iv-tubulin-antibody-epr16775-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)Immunohistochemical analysis of paraffin embedded Mouse hippocampus tissue labeling beta IV Tubulin with ab179504 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Cytoplasm staining is observed on neurons of mouse hippocampus.
Negative control used PBS instead of ab179504, followed by prediluted HRP Polymer for Rabbit/Mouse IgG.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)](https://www.abcam.com/ps/products/179/ab179504/Images/ab179504-228639-anti-beta-iv-tubulin-antibody-epr16775-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)Immunohistochemical analysis of paraffin-embedded Rat thalamus tissue labeling beta IV Tubulin with ab179504 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Cytoplasm staining is observed on neurons of rat thalamus.
Negative control used PBS instead of ab179504, followed by prediluted HRP Polymer for Rabbit/Mouse IgG.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)](https://www.abcam.com/ps/products/179/ab179504/Images/ab179504-230202-anti-beta-iv-tubulin-antibody-epr16775-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling beta IV Tubulinwith ab179504 at 1/20 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is rabbit monoclonal IgG (black line).
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![Anti-beta IV Tubulin antibody [EPR16775] (ab179504)](https://www.abcam.com/ps/products/179/ab179504/Images/ab179504-9-benefits-of-recombinant-antibodies.png)
Anti-beta IV Tubulin antibody [EPR16775] (ab179504)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (5)
ab179504 has been referenced in 5 publications.
- Sun SH et al. A Mouse Model of SARS-CoV-2 Infection and Pathogenesis. Cell Host Microbe 28:124-133.e4 (2020). PubMed: 32485164
- Zhu N et al. Morphogenesis and cytopathic effect of SARS-CoV-2 infection in human airway epithelial cells. Nat Commun 11:3910 (2020). PubMed: 32764693
- Bi B et al. Quantitative Proteomic Analysis Reveals Impaired Axonal Guidance Signaling in Human Postmortem Brain Tissues of Chronic Traumatic Encephalopathy. Exp Neurobiol 28:362-375 (2019). PubMed: 31308796
- Royston L et al. Viral chimeras decrypt the role of enterovirus capsid proteins in viral tropism, acid sensitivity and optimal growth temperature. PLoS Pathog 14:e1006962 (2018). PubMed: 29630666
- Essaidi-Laziosi M et al. Propagation of respiratory viruses in human airway epithelia reveals persistent virus-specific signatures. J Allergy Clin Immunol 141:2074-2084 (2018). ICC/IF . PubMed: 28797733
