Overview

  • Product name

    Anti-beta IV Tubulin antibody [EPR16776]
    See all beta IV Tubulin primary antibodies
  • Description

    Rabbit monoclonal [EPR16776] to beta IV Tubulin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human beta IV Tubulin aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: P04350

  • Positive control

    • WB: Human fetal liver lysate, Human fetal heart lysate, Human fetal kidney lysate, Human cerebellum lysate, HeLa whole cell lysate, Jurkat whole cell lysate, Neuro-2a whole cell lysate, Mouse brain lysate, Mouse spleen lysate , Rat brain lysate, Rat spleen lysate, C6 whole cell lysate, Raw264.7 whole cell lysate, PC-12 whole cell lysate, NIH/3T3 whole cell lysate. IHCP: Mouse cerebrum and Mouse testis tissues. IF/ICC and FC: HeLa and C6 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab179509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/150.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC application is recommended for mouse only.

WB 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ICC/IF 1/500.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • Sequence similarities

    Belongs to the tubulin family.
  • Domain

    The highly acidic C-terminal region may bind cations such as calcium.
  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta 4 antibody
    • Beta 4 tubulin antibody
    • beta 5 antibody
    • beta four tubulin antibody
    • Dystonia 4 torsion (autosomal dominant) antibody
    • MC1R antibody
    • TBB4_HUMAN antibody
    • TUB B4 antibody
    • TUBB 4 antibody
    • tubb4 antibody
    • TUBB4A antibody
    • TUBB5 antibody
    • Tubulin 5 beta antibody
    • Tubulin beta 3 antibody
    • Tubulin beta 4 antibody
    • Tubulin beta 4 chain antibody
    • Tubulin beta 4A class IVa antibody
    • Tubulin beta 5 antibody
    • Tubulin beta IV antibody
    • Tubulin beta-4 chain antibody
    see all

Images

  • Immunofluorescent analysis of 100% methanol fixed C6 (Rat glial tumor cell line ) labeling beta IV Tubulin with ab179509 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution(green).

    Cytoplasm staining on C6 cell line is observed.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [EPR16776] -Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).
    The negative controls are as follows:-
    -ve control 1 : ab179509 at 1/500 dilution followed by ab150120 at 1/1000 dilution.
    -ve control 2.: ab7291  at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

  • All lanes : Anti-beta IV Tubulin antibody [EPR16776] (ab179509) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa


    Exposure time: 30 seconds


    Blocking and diluting buffer was 5% NFDM /TBST

  • All lanes : Anti-beta IV Tubulin antibody [EPR16776] (ab179509) at 1/1000 dilution

    Lane 1 : Human cerebellum lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa


    Exposure time: 30 seconds


    Blocking and diluting buffer was 5% NFDM /TBST.

    Exposure time - Lane 1: 1 second; Lanes 2,3 and 4: 30 seconds

  • All lanes : Anti-beta IV Tubulin antibody [EPR16776] (ab179509) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Rat spleen lysate
    Lane 5 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 6 : Raw264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 8 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa


    Exposure time: 30 seconds


    Blocking and diluting buffer was 5% NFDM /TBST.
    Exposure time - Lane 1: 1 second; Lane 2: 30 seconds; Lanes 3 and 4: 3 minutes; Lanes 5, 6, 7 and 8: 30 seconds

  • Immunofluorescent analysis of 100% methanol fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling beta IV Tubulin with ab179509 at 1/500 dilution, followed by Goat Anti-Rabbit IgG  (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Cytoplasm staining on HeLa cell line is observed.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [EPR16776] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L  (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:-
    -ve control 1 - ab179509 at 1/500  followed by ab150120 at 1/1000.
    -ve control 2. - ab7291 at 1/1000 followed by ab150077  at 1/1000.

  • Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling beta IV Tubulin with ab179509 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Mouse cerebrum is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody,secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling beta IV Tubulin with ab179509 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Mouse testis is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody,secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling beta IV Tubulin with ab179509 at 1/150 dilution (red) compared with a Rabbit IgG,monoclonal [EPR16776]- Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed C6 (Rat glial tumor cell line) labeling beta IV Tubulin with ab179509 at 1/150 (red) compared with a Rabbit IgG,monoclonal [EPR16776] -Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

References

This product has been referenced in:

  • Child KM  et al. The Neuroregenerative Capacity of Olfactory Stem Cells Is Not Limitless: Implications for Aging. J Neurosci 38:6806-6824 (2018). Read more (PubMed: 29934351) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human tracheal cultures)
Permeabilization
Yes - Saponin
Specification
human tracheal cultures
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

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Verified customer

Submitted Aug 18 2017

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