RecombinantRabMAb

Recombinant Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (ab238433)

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Flow Cytometry - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (ab238433)
  • Flow Cytometry - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (ab238433)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (ab238433)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (ab238433)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (ab238433)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (ab238433)
  • Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (ab238433)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16776] to beta IV Tubulin - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free
    See all beta IV Tubulin primary antibodies

  • Description

    Rabbit monoclonal [EPR16776] to beta IV Tubulin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human beta IV Tubulin aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: P04350

  • Positive control

    • IHC-P: Mouse testis and cerebrum tissues. ICC/IF: HeLa and C6 cells. Flow Cyt: HeLa and C6 cells.

  • General notes

    Ab238433 is the carrier-free version of ab179509. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238433 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab238433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC application is recommended for mouse only.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

  • Sequence similarities

    Belongs to the tubulin family.

  • Domain

    The highly acidic C-terminal region may bind cations such as calcium.

  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.

  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt

  • Database links

    • Alternative names

      • Beta 4 antibody
      • Beta 4 tubulin antibody
      • beta 5 antibody
      • beta four tubulin antibody
      • Dystonia 4 torsion (autosomal dominant) antibody
      • MC1R antibody
      • TBB4_HUMAN antibody
      • TUB B4 antibody
      • TUBB 4 antibody
      • tubb4 antibody
      • TUBB4A antibody
      • TUBB5 antibody
      • Tubulin 5 beta antibody
      • Tubulin beta 3 antibody
      • Tubulin beta 4 antibody
      • Tubulin beta 4 chain antibody
      • Tubulin beta 4A class IVa antibody
      • Tubulin beta 5 antibody
      • Tubulin beta IV antibody
      • Tubulin beta-4 chain antibody
      see all

    Images

    • Flow Cytometry - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (ab238433)
      Flow Cytometry - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (ab238433)

      Flow cytometric analysis of 4% paraformaldehyde-fixed C6 (Rat glial tumor cell line) labeling beta IV Tubulin with ab179509 at 1/150 (red) compared with a Rabbit IgG,monoclonal [EPR16776] -Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179509).

    • Flow Cytometry - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (ab238433)
      Flow Cytometry - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (ab238433)

      Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling beta IV Tubulin with ab179509 at 1/150 dilution (red) compared with a Rabbit IgG,monoclonal [EPR16776]- Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179509).

    • Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (ab238433)
      Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (ab238433)

      Immunofluorescent analysis of 100% methanol fixed C6 (Rat glial tumor cell line ) labeling beta IV Tubulin with ab179509 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution(green).

      Cytoplasm staining on C6 cell line is observed.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with Anti-alpha Tubulin antibody [EPR16776] -Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).
      The negative controls are as follows:-
      -ve control 1 : ab179509 at 1/500 dilution followed by ab150120 at 1/1000 dilution.
      -ve control 2.: ab7291  at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179509).

    • Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (ab238433)
      Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (ab238433)

      Immunofluorescent analysis of 100% methanol fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling beta IV Tubulin with ab179509 at 1/500 dilution, followed by Goat Anti-Rabbit IgG  (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Cytoplasm staining on HeLa cell line is observed.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with Anti-alpha Tubulin antibody [EPR16776] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L  (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

      The negative controls are as follows:-
      -ve control 1 - ab179509 at 1/500  followed by ab150120 at 1/1000.
      -ve control 2. - ab7291 at 1/1000 followed by ab150077  at 1/1000.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179509).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (ab238433)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (ab238433)

      Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling beta IV Tubulin with ab179509 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Mouse testis is observed. Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary antibody,secondary antibody is ab97051 at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179509).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (ab238433)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (ab238433)

      Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling beta IV Tubulin with ab179509 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Mouse cerebrum is observed. Counter stained with Hematoxylin.

      Negative control: Used PBS instead of primary antibody,secondary antibody is ab97051 at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179509).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (ab238433)
      Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (ab238433)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab238433? Please let us know so that we can cite the reference in this datasheet.

    ab238433 has not yet been referenced specifically in any publications.

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