Overview

  • Product name

    Anti-beta IV Tubulin antibody [ONS.1A6] (HRP)
    See all beta IV Tubulin primary antibodies
  • Description

    Mouse monoclonal [ONS.1A6] to beta IV Tubulin (HRP)
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Hamster, Cow
  • Immunogen

    Synthetic peptide corresponding to beta IV Tubulin (C terminal) conjugated to bovine serum albumin.

  • Positive control

    • WB: HeLa, HEK293 and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab204454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • Sequence similarities

    Belongs to the tubulin family.
  • Domain

    The highly acidic C-terminal region may bind cations such as calcium.
  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta 4 antibody
    • Beta 4 tubulin antibody
    • beta 5 antibody
    • beta four tubulin antibody
    • Dystonia 4 torsion (autosomal dominant) antibody
    • MC1R antibody
    • TBB4_HUMAN antibody
    • TUB B4 antibody
    • TUBB 4 antibody
    • tubb4 antibody
    • TUBB4A antibody
    • TUBB5 antibody
    • Tubulin 5 beta antibody
    • Tubulin beta 3 antibody
    • Tubulin beta 4 antibody
    • Tubulin beta 4 chain antibody
    • Tubulin beta 4A class IVa antibody
    • Tubulin beta 5 antibody
    • Tubulin beta IV antibody
    • Tubulin beta-4 chain antibody
    see all

Images

  • All lanes : Anti-beta IV Tubulin antibody [ONS.1A6] (HRP) (ab204454) at 1/5000 dilution

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab204454 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab204454 has not yet been referenced specifically in any publications.

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