Overview

  • Product name
    Anti-Beta Lactamase antibody [8A5.A10]
  • Description
    Mouse monoclonal [8A5.A10] to Beta Lactamase
  • Host species
    Mouse
  • Specificity
    This antibody specifically recognizes TEM type beta lactamases.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Recombinant full length protein corresponding to Beta Lactamase.
    Sequence:

    MSIQHFRVAL IPFFAAFCLP VFAHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW

  • General notes

    Dilute in PBS or medium which is identical to that used in the assay system.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab12251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 10 - 20 µg/ml.
WB Use a concentration of 10 µg/ml. Predicted molecular weight: 31.5 kDa. (31.5kDa is the molecular weight of the unprocessed precursor.)

Target

  • Relevance
    The beta lactam antibiotics (penicillins and cephalosporins) are the most frequently used antimicrobial agents. All of the beta lactams are structurally related through the presence of a core beta lactam ring. Bacterial resistance to beta lactams continues to increase, primarily due to the production of beta lactamases. Beta lactamases catalyze the hydrolysis of the beta lactam bond, which destroys antibacterial activity. Bacteria that produce TEM type or SHV type beta lactamases have point mutations in structural genes that have extended the substrate specificity of these beta lactamases. As a result, many of the beta lactamase producing Gram negative pathogens have become multidrug resistant.
  • Alternative names
    • AmpA antibody
    • AmpC antibody
    • Cephalosporinase antibody

References

This product has been referenced in:
  • Sutherland GA  et al. Probing the quality control mechanism of the Escherichia coli twin-arginine translocase with folding variants of a de novo-designed heme protein. J Biol Chem 293:6672-6681 (2018). WB . Read more (PubMed: 29559557) »
  • Liu Y  et al. Transcriptomic dissection of the horizontally acquired response regulator EsrB reveals its global regulatory roles in the physiological adaptation and activation of T3SS and the cognate effector repertoire in Edwardsiella piscicida during infection toward turbot. Virulence 8:1355-1377 (2017). Read more (PubMed: 28441105) »
See all 18 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for your enquiry.

ab12251 and ab12252 were derived from mice that were immunized with the full-length beta lactamse. The immunogen of ab12251 was recombinant TEM-1 beta-lactamase while ab12252 was native Type IV beta-lactamase from Enterobacter cloacae.

The lactamases appear to be well-conserved, and the antibodies ab97678, ab12252 ab12251 are likely to react with a variety of them. They may possibly recognize your sequence but the epitopes they recognize have not been mapped, so we cannot guarantee these antibodies are all recognize different epitopes.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

ab12251's immunogen is 5'-His-tagged E.coli TEM-1 beta lactamase therefore this antibody is specific to TEM-1 beta lactamases. I have compared the sequence similarity between TEM-1 beta lactamase and ab104926 and only found 10% sequence similarity so it is unlikely that this antibody will detect this protein.

However, I have found another protein, beta lactamase TEM precursor protein, ab67672 (https://www.abcam.com/ab67672) which has a 99.6% sequence similarity with TEM-1 beta lactamase. Therefore, this protein is likely to be detected by ab12251.

If you would like a proforma for these two products, please let me know and I will be more than happy to do so.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Thank you for contacting us on Friday.

As discussed over the phone, ab12251 and ab97678 are both mouse monoclonals against beta-lactamase. Whilst I cannot find any reference of ab97678 being used in detecting beta-galctosidase expressed in Legionalla, we have a reference where ab12251 has been used for this purpose. A link to this reference can be accessed from below:

Zhu W et al. Comprehensive Identification of Protein Substrates of the Dot/Icm Type IV Transporter of Legionella pneumophila. PLoS One 6:e17638 (2011). PubMed: 21408005

There seems to relatively little background produced in this system with this antibody, and the background that is there could probably be improved on by reducing the amount of protein loaded and optimising the blocking and incubation conditions.

As discussed over the phone, if you were to want to try either ab12251 and ab97678 to detect beta-lactamase in your system, you would be eligible forour testing discount system. This is usually only valid where the application or species for which you want to use the antibody for have not been tested (and are therefore not stated on the datasheet). In this case, the sample and application would be covered by the Abpromise and would therefore not be valid for the testing discount. However, as there are no images on the datasheets of either of these antibodies, we would be willing to extend the testing discount scheme to include a reward for providing us with images of the antibodies being used.

This would involve you purchasing ab12251or ab97678. Testing it in western blotting, then sharing the results obtained through an Abreview (including an image of the results). You would then be entitled to a free primary antibody of your choice from our catalogue. The only limitation is that the antibody needs to be tested, the Abreview submitted, and the "free" antibody claimed within 4 months. More information on this offer can be foundfrom the following link:

https://www.abcam.com/collaboratordiscount

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issuedprior to purchasing the antibody to be tested.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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Answer

Thank you for contacting us. The lactamases appear to be well-conserved, and the antibodies ab97678 and ab12251 are likely to react with a variety of them. They may possibly recognize your sequence but the epitopes they recognize have not been mapped, so we cannot guarantee this. Regarding the sizes and prices, the 500ug size is the only one available for ab12251, and the 200ug size is the only size available for ab97678. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

To the best of our knowledge, this antibody recognizes all TEM-type beta lactamases. Data is limited to our own work with TEM-1 and reports from customers who have worked with various strains of E. coli. To date, we have not been informed by any of our customers of TEM-type beta lactamases that were not recognized by this antibody, but, presumably, not all relevant strains have been investigated. Good luck with your research,

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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