Key features and details
- Mouse monoclonal [8A5.A10] to Beta Lactamase
- Suitable for: ELISA, WB
- Reacts with: Escherichia coli
- Isotype: IgG1
Product nameAnti-Beta Lactamase antibody [8A5.A10]
DescriptionMouse monoclonal [8A5.A10] to Beta Lactamase
SpecificityThis antibody specifically recognizes TEM type beta lactamases.
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Escherichia coli
Recombinant full length protein corresponding to Beta Lactamase.
MSIQHFRVAL IPFFAAFCLP VFAHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW
Dilute in PBS or medium which is identical to that used in the assay system.
This product was changed from ascites to tissue culture supernatant on 19/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions please do not hesitate to contact our scientific support team.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Concentration information loading...
PurityProtein G purified
Purification notesPurified from TCS
Our Abpromise guarantee covers the use of ab12251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 10 - 20 µg/ml.|
|WB||Use a concentration of 10 µg/ml. Predicted molecular weight: 31.5 kDa. (31.5kDa is the molecular weight of the unprocessed precursor.)|
RelevanceThe beta lactam antibiotics (penicillins and cephalosporins) are the most frequently used antimicrobial agents. All of the beta lactams are structurally related through the presence of a core beta lactam ring. Bacterial resistance to beta lactams continues to increase, primarily due to the production of beta lactamases. Beta lactamases catalyze the hydrolysis of the beta lactam bond, which destroys antibacterial activity. Bacteria that produce TEM type or SHV type beta lactamases have point mutations in structural genes that have extended the substrate specificity of these beta lactamases. As a result, many of the beta lactamase producing Gram negative pathogens have become multidrug resistant.
- AmpA antibody
- AmpC antibody
- Cephalosporinase antibody
ab12251 has been referenced in 23 publications.
- Ballard A et al. The fitness consequences of synonymous mutations in Escherichia coli: Experimental evidence for a pleiotropic effect of translational selection. Gene 694:111-120 (2019). PubMed: 30738968
- Singh R et al. A ß-lactamase-producing plasmid from Neisseria gonorrhoeae carrying a unique 6 bp deletion in blaTEM-1 encoding a truncated 24 kDa TEM-1 penicillinase that hydrolyses ampicillin slowly. J Antimicrob Chemother 74:2904-2912 (2019). PubMed: 31335939
- Zhao Q et al. Integrin but not CEACAM receptors are dispensable for Helicobacter pylori CagA translocation. PLoS Pathog 14:e1007359 (2018). PubMed: 30365569
- Sutherland GA et al. Probing the quality control mechanism of the Escherichia coli twin-arginine translocase with folding variants of a de novo-designed heme protein. J Biol Chem 293:6672-6681 (2018). WB . PubMed: 29559557
- Tooke FJ et al. A unifying mechanism for the biogenesis of membrane proteins co-operatively integrated by the Sec and Tat pathways. Elife 6:N/A (2017). PubMed: 28513434