Product nameBeta Lactamase Inhibitor Screening Assay Kit (Colorimetric)
See all Beta lactamase kits
Sample typeInhibitor compounds
Assay typeEnzyme activity
Beta Lactamase Inhibitor Screening Assay Kit (Colorimetric) (ab197009) is a reliable and sensitive assay suitable for high throughput screening of Beta Lactamase (β-Lactamase) inhibitors. The assay is based on the ability of the enzyme Beta Lactamase to hydrolyze the substrate Nitrocefin, a chromogenic cephalosporin, which results in the generation of a colored product detectable at OD=490 nm. The amount of color produced is directly proportional to the amount of beta-lactamase activity. In the presence of Beta-Lactamase inhibitors, such as clavulanic acid, the rate of substrate hydrolysis will decrease resulting in a decrease in the production of colored analyte.
This kit provides a simple test for screening, studying and/or characterizing Beta-Lactamse inhibitors for efficient treatment of antibiotic resistant bacterial infections.
Beta Lactamase (β-Lactamase, βL), (EC 220.127.116.11), is a bacterial enzyme that hydrolyzes the β-Lactam ring, a four-carbon ring, the building block of β-Lactam antibiotics. β-Lactams inhibit transpeptidases, enzymes that participate in the biosynthesis of bacterial cell walls. Penams, Carbapenems, Cephems, Clavams, Oxacephems and Monobactams have been widely used for the treatment of bacterial infections since the discovery of penicillin, the first β-Lactam antibiotic, and its bactericidal effect more than eighty years ago. However, an alarming number of cases of β-Lactam antibiotic-resistant bacterial strains have been reported. The resistance is due to the production of β-Lactamase, responsible for diminishing the β-Lactam antibiotic potency. Inhibition of β-Lactamase activity has therefore become an urgent target in the treatment of bacterial infections displaying β-Lactam resistance. Several β-Lactam derivatives have been reported as β-Lactamase inhibitors; however,only clavulanic acid, sulbactam and tazobactam have reached clinical importance.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests Beta Lactamase 1 vial Beta Lactamase Assay Buffer 1 x 25ml Inhibitor Control 1 x 50µl Nitrocefin 1 x 0.1ml
ab197009 has not yet been referenced specifically in any publications.