Overview

  • Product name
    beta-Lapachone, Topoisomerase I inhibitor
  • Description
    Topoisomerase I inhibitor
  • Biological description
    Topoisomerase I inhibitor. Inhibits topoisomerase I induced DNA cleavage. Induces apoptosis via a p53 independent mechanism. Shows antitumor, antiviral, antibacterial, antifungal and wound healing activities.
  • Purity
    > 98%

Properties

Applications

Our Abpromise guarantee covers the use of ab141097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab18209 staining p21 in serum starved PC-3 cells treated with ß-lapachone (ab141097), by ICC/IF. Increase of p21 nuclear expression correlates with increased concentration of ß-lapachone, as described in literature.
    The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab141097 (ß-lapachone) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab18209 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:
  • Wu YT  et al. ß-Lapachone induces heart morphogenetic and functional defects by promoting the death of erythrocytes and the endocardium in zebrafish embryos. J Biomed Sci 18:70 (2011). Read more (PubMed: 21936955) »
  • Planchon SM  et al. Beta-lapachone-mediated apoptosis in human promyelocytic leukemia (HL-60) and human prostate cancer cells: a p53-independent response. Cancer Res 55:3706-11 (1995). Read more (PubMed: 7641180) »
  • Li CJ  et al. beta-Lapachone, a novel DNA topoisomerase I inhibitor with a mode of action different from camptothecin. J Biol Chem 268:22463-8 (1993). Read more (PubMed: 8226754) »

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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