Product nameAnti-beta-phosphoglucomutase antibody (Biotin)
See all beta-phosphoglucomutase primary antibodies
DescriptionGoat polyclonal to beta-phosphoglucomutase (Biotin)
Tested applicationsSuitable for: Immunomicroscopy, WB, ELISA, Dot blotmore details
Species reactivityReacts with Lactococcus lacti
Full length protein corresponding to beta-phosphoglucomutase.
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride
BSA is IgG and Protease free
Concentration information loading...
Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Our Abpromise guarantee covers the use of ab34541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/4000 - 1/20000. This product has been assayed against 1.0 ug of beta Phosphoglucomutase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 62 kDa.
Suitable for antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Relevanceß-phosphoglucomutase is an enzyme that transfers a phosphoryl group on a glucose monomer from the 1' to the 6' position in the forward direction or the 6' to the 1' position in the reverse. Specifically, it converts ß-D-glucose-1-phosphate to ß-D-glucose-6-phosphate. This enzyme participates in both the breakdown and synthesis of glucose. Maltose metabolism in Lactococcus lactis involves the conversion of beta-glucose 1-phosphate to glucose 6-phosphate, a reaction which is reversibly catalysed by a maltose-inducible and glucose-repressible beta-phosphoglucomutase (beta-PGM). Alpha-PGM is expressed constitutively. ß-phosphoglucomutase is a member of the haloacid dehalogenase superfamily of hydrolase enzymes. The enzyme from Lactococcus lactis has been extensively characterised including a remarkable crystal structure which traps the pentacoordinate transition state.
- Beta PGM antibody
- Beta-phosphoglucomutase antibody
- Glucose phosphomutase 1 antibody
ab34541 has not yet been referenced specifically in any publications.