Anti-beta Sarcoglycan antibody (ab83699)
Key features and details
- Rabbit polyclonal to beta Sarcoglycan
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-beta Sarcoglycan antibody
See all beta Sarcoglycan primary antibodies -
Description
Rabbit polyclonal to beta Sarcoglycan -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog -
Immunogen
Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (
FSTDYETHEFHLPSGVKSLNVQKASTERITSNATSDLNIKVDGRAIVRGN
) of Human beta Sarcoglycan (NP_000223) -
Positive control
- WB: Human heart and human fetal heart lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab83699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
Target
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Function
Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix. -
Tissue specificity
Highest expression in heart and skeletal muscle. Low expression in brain, kidney, placenta, pancreas and lung. High expression in fetal brain. Also found in fetal lung, kidney and liver. -
Involvement in disease
Limb-girdle muscular dystrophy 2E -
Sequence similarities
Belongs to the sarcoglycan beta/delta/gamma/zeta family. -
Post-translational
modificationsDisulfide bonds are present. -
Cellular localization
Cell membrane, sarcolemma. Cytoplasm, cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 535372 Cow
- Entrez Gene: 6443 Human
- Entrez Gene: 24051 Mouse
- Entrez Gene: 100009208 Rabbit
- Entrez Gene: 680229 Rat
- Omim: 600900 Human
- SwissProt: A6QP70 Cow
- SwissProt: Q16585 Human
see all -
Alternative names
- 43 kDa dystrophin associated glycoprotein antibody
- 43 kDa dystrophin-associated glycoprotein antibody
- 43DAG antibody
see all
Images
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Anti-beta Sarcoglycan antibody (ab83699) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human heart lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Gel concentration: 12% -
Anti-beta Sarcoglycan antibody (ab83699) at 1 µg/ml + Human fetal heart lysate at 10 µg
Predicted band size: 35 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab83699 has been referenced in 1 publication.
- Scano M et al. CFTR corrector C17 is effective in muscular dystrophy, in vivo proof of concept in LGMDR3. Hum Mol Genet 31:499-509 (2022). PubMed: 34505136