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    beta-subunit-cholera-toxin-antibody-263-ab35988.pdf

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Microbiology Toxin Bacterial Toxin Cholera Toxin
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Anti-beta subunit Cholera Toxin antibody [2/63] (ab35988)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (5)

Product price, shipping and contact information

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Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Mouse monoclonal [2/63] to beta subunit Cholera Toxin
  • Suitable for: IHC (PFA fixed), ELISA
  • Reacts with: Species independent
  • Isotype: IgG1

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Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

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Overview

  • Product name

    Anti-beta subunit Cholera Toxin antibody [2/63]
    See all beta subunit Cholera Toxin primary antibodies
  • Description

    Mouse monoclonal [2/63] to beta subunit Cholera Toxin
  • Host species

    Mouse
  • Specificity

    ab35988 is reactive with the beta chain of the V. cholerae toxin.
  • Tested applications

    Suitable for: IHC (PFA fixed), ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Native Vibrio cholera Toxin

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    2/63
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Microbiology
    • Toxin
    • Bacterial Toxin
    • Cholera Toxin

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

Our Abpromise guarantee covers the use of ab35988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed) Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • Cellular localization

    Secreted
  • Alternative names

    • Cholera enterotoxin B chain antibody
    • Cholera enterotoxin beta chain antibody
    • Cholera enterotoxin gamma chain antibody
    • Cholera enterotoxin subunit B antibody
    • Cholera toxin B protein antibody
    • Choleragenoid antibody
    • CTX B antibody
    • CTXB antibody
    • TOX B antibody
    • TOXB antibody
    • VC1456 antibody
    see all

Protocols

  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (5)

    Publishing research using ab35988? Please let us know so that we can cite the reference in this datasheet.

    ab35988 has been referenced in 5 publications.

    • Ellender TJ  et al. Embryonic progenitor pools generate diversity in fine-scale excitatory cortical subnetworks. Nat Commun 10:5224 (2019). PubMed: 31745093
    • Dautan D  et al. Segregated cholinergic transmission modulates dopamine neurons integrated in distinct functional circuits. Nat Neurosci 19:1025-33 (2016). IHC ; Rat . PubMed: 27348215
    • Cameron D  et al. The organisation of spinoparabrachial neurons in the mouse. Pain 156:2061-71 (2015). PubMed: 26101837
    • Baseer N  et al. Selective innervation of NK1 receptor-lacking lamina I spinoparabrachial neurons by presumed nonpeptidergic Ad nociceptors in the rat. Pain N/A:N/A (2014). IHC-FoFr . PubMed: 25168670
    • Oakman SA  et al. Distribution of pontomesencephalic cholinergic neurons projecting to substantia nigra differs significantly from those projecting to ventral tegmental area. J Neurosci 15:5859-69 (1995). PubMed: 7666171

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-beta subunit Cholera Toxin antibody [2/63]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Chlamydomonas reinhardtii Cell lysate - whole cell (n/a)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    n/a
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 11 2018

    Question

    Thank you for your enquiry. Our antibody to Beta Subunit Cholera Toxin (ab34992) should recognize the beta subunit in both monomeric and pentameric forms. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 12 2010

    Answer

    Thank you for your enquiry. Our antibody to Beta Subunit Cholera Toxin (ab35988) should recognize the beta subunit in both monomeric and pentameric forms. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Feb 12 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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