Anti-beta subunit Cholera Toxin antibody (ab34992)

Submit an Abreview Q&A (5)Specific References (10)

Overview

  • Product name
    Anti-beta subunit Cholera Toxin antibody
    See all beta subunit Cholera Toxin primary antibodies
  • Description
    Rabbit polyclonal to beta subunit Cholera Toxin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, Purification, IHC-FoFr, Immunodiffusion, WBmore details
  • Species reactivity
    Reacts with: Other species
  • Immunogen

    Purified Choleragenoid.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purification notes
    >95% pure
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/200 - 1/2000.
Purification Use at an assay dependent concentration. PubMed: 23355891
IHC-FoFr 1/1000. PubMed: 19406878
Immunodiffusion Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. PubMed: 24648517

Target

  • Cellular localization
    Secreted
  • Alternative names
    • Cholera enterotoxin B chain antibody
    • Cholera enterotoxin beta chain antibody
    • Cholera enterotoxin gamma chain antibody
    • Cholera enterotoxin subunit B antibody
    • Cholera toxin B protein antibody
    • Choleragenoid antibody
    • CTX B antibody
    • CTXB antibody
    • TOX B antibody
    • TOXB antibody
    • VC1456 antibody
    see all

References

This product has been referenced in:
  • Cervin J  et al. GM1 ganglioside-independent intoxication by Cholera toxin. PLoS Pathog 14:e1006862 (2018). Read more (PubMed: 29432456) »
  • Shvets E  et al. Dynamic caveolae exclude bulk membrane proteins and are required for sorting of excess glycosphingolipids. Nat Commun 6:6867 (2015). Human . Read more (PubMed: 25897946) »

See all 10 Publications for this product

Publishing research using ab34992? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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5 reviews or Q&A

10
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I have enclosed the requested blots as per our previous conversation. Please let me know If there is anything else I could provide you. I would really like the issue resolved. I want to clearly state the new lot of antibody is not working as the previo...

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Thank you for contacting us. Your credit note ID is CNXXXXX. I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasi...

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I have enclosed the requested blots as per our previous conversation. Please let me know If there is anything else I could provide you. I would really like the issue resolved. I want to clearly state the new lot of antibody is not working as the previo...

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Thank you for getting back to me and for providing some further details of the experiments. I am very sorry to hear that you are dissatisfied with the quality of this product. According to the originator of ab34992, they tested the suitability ...

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Hi, I ordered Anti-beta subunit Cholera Toxin antibody(ab34992). lot no GR6241-3. I see signal in western blot analysis. But when I used the antibody for Immunoprecipitation, I don't see any binding with my antigen.I am trying to elute the antigen that...

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Thank you for your enquiry regarding ab34992. As my colleague has informed you previously, this antibody has been tested for IP in gel diffusion techniques. The originator, unfortunately, do not have any supporting references. However, they suggest usi...

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Would like pre-immune serum from the rabbit that produced ab34992.

Thank you for your call yesterday and for your patience while I have been in touch with the lab about ab34992. Unfortunately the pre-immune serum is not available for this antibody. However, we do have rabbit serum and rabbit IgG isotype contro...

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I have a question about ab34992 beta subunit Cholera Toxin antibody. Can ab34992 detect both monometric and pentameric forms of Cholera Toxin beta subunit?

Thank you for your enquiry. Our antibody to Beta Subunit Cholera Toxin (ab34992) should recognize the beta subunit in both monomeric and pentameric　forms. I hope this information helps, please do not hesitate to contact us if you need any more advi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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