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  1. Link

    beta-subunit-cholera-toxin-antibody-ab34992.pdf

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Microbiology Toxin Bacterial Toxin Cholera Toxin
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Anti-beta subunit Cholera Toxin antibody (ab34992)

  • Datasheet
  • SDS
Submit a review Q&A (5)References (20)

Product price, shipping and contact information

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Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Rabbit polyclonal to beta subunit Cholera Toxin
  • Suitable for: ELISA, Immunodiffusion
  • Isotype: IgG

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Overview

  • Product name

    Anti-beta subunit Cholera Toxin antibody
    See all beta subunit Cholera Toxin primary antibodies
  • Description

    Rabbit polyclonal to beta subunit Cholera Toxin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, Immunodiffusionmore details
  • Species reactivity

    Reacts with: Vibrio cholerae
  • Immunogen

    Full length native protein (purified) corresponding to beta subunit Cholera Toxin. Purified choleragenoid.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    >95% pure
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Toxin
    • Bacterial Toxin
    • Cholera Toxin

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab34992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
1/200 - 1/2000.
Immunodiffusion
Use at an assay dependent concentration.
Notes
ELISA
1/200 - 1/2000.
Immunodiffusion
Use at an assay dependent concentration.

Target

  • Cellular localization

    Secreted
  • Alternative names

    • Cholera enterotoxin B chain antibody
    • Cholera enterotoxin beta chain antibody
    • Cholera enterotoxin gamma chain antibody
    • Cholera enterotoxin subunit B antibody
    • Cholera toxin B protein antibody
    • Choleragenoid antibody
    • CTX B antibody
    • CTXB antibody
    • TOX B antibody
    • TOXB antibody
    • VC1456 antibody
    see all

Protocols

  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (20)

Publishing research using ab34992? Please let us know so that we can cite the reference in this datasheet.

ab34992 has been referenced in 20 publications.

  • Song SY  et al. The CSF-Contacting Nucleus Receives Anatomical Inputs From the Cerebral Cortex: A Combination of Retrograde Tracing and 3D Reconstruction Study in Rat. Front Neuroanat 14:600555 (2020). PubMed: 33328908
  • Sieveritz B & Arbuthnott GW Prelimbic cortical targets of ventromedial thalamic projections include inhibitory interneurons and corticostriatal pyramidal neurons in the rat. Brain Struct Funct 225:2057-2076 (2020). PubMed: 32661702
  • Song SY  et al. Novel Projections to the Cerebrospinal Fluid-Contacting Nucleus From the Subcortex and Limbic System in Rat. Front Neuroanat 14:57 (2020). PubMed: 32973466
  • Schneider NY  et al. Centrifugal projections to the main olfactory bulb revealed by transsynaptic retrograde tracing in mice. J Comp Neurol 528:1805-1819 (2020). PubMed: 31872441
  • Lan H  et al. Impaired mucociliary motility enhances antigen-specific nasal IgA immune responses to a cholera toxin-based nasal vaccine. Int Immunol 32:559-568 (2020). PubMed: 32347929
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-5 of 5 Abreviews or Q&A

Question

I have enclosed the requested blots as per our previous conversation. Please let me know If there is anything else I could provide you. I would really like the issue resolved. I want to clearly state the new lot of antibody is not working as the previous one. Thank you. Regards,

Read More

Abcam community

Verified customer

Asked on Dec 21 2011

Answer

Thank you for contacting us. Your credit note ID is CNXXXXX. I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Read More

Abcam Scientific Support

Answered on Dec 21 2011

Question

I have enclosed the requested blots as per our previous conversation. Please let me know If there is anything else I could provide you. I would really like the issue resolved. I want to clearly state the new lot of antibody is not working as the previous one. Thank you.     Regards,    

Read More

Abcam community

Verified customer

Asked on Nov 02 2011

Answer

Thank you for getting back to me and for providing some further details of the experiments. I am very sorry to hear that you are dissatisfied with the quality of this product. According to the originator of ab34992, they tested the suitability of this product for IP in gel diffusion techniques. At this stage, they do not have any supporting published references yet. As soon as we have more information, we will update the on-line datasheet accordingly. I could certainly arrange a credit note or a refund for you as you requested. Could you please confirm your Abcam Order Number (or your Purchase Order Number), the date or purchase and the name of your Institute. I look forward to hearing from you

Read More

Abcam Scientific Support

Answered on Nov 02 2011

Question

Hi, I ordered Anti-beta subunit Cholera Toxin antibody(ab34992). lot no GR6241-3. I see signal in western blot analysis. But when I used the antibody for Immunoprecipitation, I don't see any binding with my antigen.I am trying to elute the antigen that binds with antibody. The strange thing is I have previously used the same antibody to immunoprecipitate my antigen. Since I ran out of antibody, I ordered  new lot and it doesn't work. What would you advise me to do ? Thanks. regards    

Read More

Abcam community

Verified customer

Asked on Oct 17 2011

Answer

Thank you for your enquiry regarding ab34992. As my colleague has informed you previously, this antibody has been tested for IP in gel diffusion techniques. The originator, unfortunately, do not have any supporting references. However, they suggest using the antibody at a concentration of around 10 to 50µg/ml for IP. Also it is advisable to titrate around these suggested concentrations in order to find the optimal dilution. Could you please provide some further details of the experiments and an image representing the results with the different bathes? I look forward to hearing from you soon. Thank you for your understanding and co-operation in this matter.    

Read More

Abcam Scientific Support

Answered on Oct 17 2011

Question

Would like pre-immune serum from the rabbit that produced ab34992.

Read More

Abcam community

Verified customer

Asked on Oct 07 2011

Answer

Thank you for your call yesterday and for your patience while I have been in touch with the lab about ab34992. Unfortunately the pre-immune serum is not available for this antibody. However, we do have rabbit serum and rabbit IgG isotype controls that should be suitable for your experiments- https://www.abcam.com/Rabbit-Serum-sterile-ab7487.html https://www.abcam.com/Rabbit-IgG-ChIP-Grade-ab37415.html Please let me know if you have any questions or if there is anything else that we can do for you, and I'll be happy to help.

Read More

Abcam Scientific Support

Answered on Oct 07 2011

Question

I have a question about ab34992 beta subunit Cholera Toxin antibody. Can ab34992 detect both monometric and pentameric forms of Cholera Toxin beta subunit?

Read More

Abcam community

Verified customer

Asked on Feb 12 2010

Answer

Thank you for your enquiry. Our antibody to Beta Subunit Cholera Toxin (ab34992) should recognize the beta subunit in both monomeric and pentameric forms. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Feb 12 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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