Key features and details
- Mouse monoclonal [6A10] to beta Synuclein
- Suitable for: IHC, WB
- Reacts with: Mouse, Rat, Cow
- Isotype: IgG1
Product nameAnti-beta Synuclein antibody [6A10]
See all beta Synuclein primary antibodies
DescriptionMouse monoclonal [6A10] to beta Synuclein
Tested applicationsSuitable for: IHC, WBmore details
Species reactivityReacts with: Mouse, Rat, Cow
- WB: Rat cortex and cerebellum, mouse cortex and cerebellum, cow cortex and cerebellum. IHC: Mouse hippocampus.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Concentration information loading...
PurityProtein G purified
Purification notesPurified from Tissue Culture supernatant.
Our Abpromise guarantee covers the use of ab277630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC||1/500 - 1/1000.
transcardial perfusion-fixed with 4% paraformaldehyde for 24 hours, free floating sections
|WB||1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).|
FunctionNon-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease.
Tissue specificityExpressed predominantly in brain; concentrated in presynaptic nerve terminals.
Sequence similaritiesBelongs to the synuclein family.
modificationsPhosphorylated. Phosphorylation by G-protein coupled receptor kinases (GRK) is more efficient than phosphorylation by CK1, CK2 and CaM-kinase II.
- Information by UniProt
- 14 kDa brain-specific protein antibody
- Beta-synuclein antibody
- Phosphoneuroprotein 14 antibody
Perfusion-fixed, free floating sections of mouse hippocampus section stained for beta Synuclein using ab277630 (green) at 1/500 dilution, and counterstained with rabbit pAb to C-terminal peptide of rat NF-L (red) at 1/5000 dilution in immunohistochemical analysis. The blue is Hoechst staining of nuclear DNA. ab277630 detects protein concentrated in synaptic regions, while the NF-L-ct antibody labels dendrites and axons of neuronal cells.
Lanes 2-7 : Anti-beta Synuclein antibody [6A10] (ab277630) at 1/1000 dilution
Lane 1 : Molecular weight ladder
Lane 2 : rat cortex
Lane 3 : rat cerebellum
Lane 4 : mouse cortex
Lane 5 : mouse cerebellum
Lane 6 : cow cortex
Lane 7 : cow cerebellum
Predicted band size: 14 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab277630 has not yet been referenced specifically in any publications.