Overview

  • Product name
    Anti-beta Synuclein antibody
    See all beta Synuclein primary antibodies
  • Description
    Rabbit polyclonal to beta Synuclein
  • Host species
    Rabbit
  • Specificity
    Less than 0.1% cross reactivity against human alpha-synuclein Cross reactivity: This antibody is known to react with human and rat. Not yet tested in other species.
  • Tested applications
    Suitable for: ICC, IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (IEPLMEPEGSYEDPPQE) corresponding to the carboxyl terminal of human beta-synuclein (108- 125) and was conjugated to diphtheria toxoid via a cystine residue added to the carboxyl terminus of the peptide. The conjugate was cross-linked by maleimidocaproyl-N-hydroxy succinimide.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

Applications

Our Abpromise guarantee covers the use of ab6165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-P 1/500 - 1/2000.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Target

  • Function
    Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease.
  • Tissue specificity
    Expressed predominantly in brain; concentrated in presynaptic nerve terminals.
  • Sequence similarities
    Belongs to the synuclein family.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation by G-protein coupled receptor kinases (GRK) is more efficient than phosphorylation by CK1, CK2 and CaM-kinase II.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
    • Alternative names
      • 14 kDa brain-specific protein antibody
      • Beta-synuclein antibody
      • Phosphoneuroprotein 14 antibody
      • PNP14 antibody
      • Sncb antibody
      • Synuclein beta antibody
      • SYUB_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody (ab6165)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody (ab6165)
      ab6165 staining beta synuclein in human occipital cortex.
      Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
      Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    References

    This product has been referenced in:
    • Lahut S  et al. Blood RNA biomarkers in prodromal PARK4 and rapid eye movement sleep behavior disorder show role of complexin 1 loss for risk of Parkinson's disease. Dis Model Mech 10:619-631 (2017). Read more (PubMed: 28108469) »
    • Helwig M  et al. Brain propagation of transduced a-synuclein involves non-fibrillar protein species and is enhanced in a-synuclein null mice. Brain 139:856-70 (2016). WB ; Mouse . Read more (PubMed: 26719384) »

    See all 4 Publications for this product

    Publishing research using ab6165? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    3 reviews or Q&A

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta Synuclein antibody

     Good
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Brain, Cortex)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric Acid
    Permeabilization
    No
    Specification
    Brain, Cortex
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
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    Abcam user community

    Verified customer

    Submitted May 23 2018

    Can you please advise me on which blocking peptide to use against rabbit anti-beta synuclein (ab6165). We are using it in human tissue for immunohistochemistry.

    Please let me know if you require any further information.

    Looking fo...

    Read More

    Thank you for your enquiry regarding ab6165.

    I regret to inform you that the blocking peptide is not available commercially.

    Apologies!

    Please see customers request below:

    Can you please advise me on which blocking peptide to use against rabbit anti-beta synuclein (ab6165). We are using it in human tissue for immunohistochemistry.

    Please let me know if you require ...

    Read More

    Thank you for your email.

    I have contacted our laboratory and inquired about this.

    I will get back to you with the updates soon.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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