For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    beta-synuclein-antibody-ab6165.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurology process Neurodegenerative disease Parkinson's disease Synuclein
Share by email

Anti-beta Synuclein antibody (ab6165)

  • Datasheet
Reviews (1)Q&A (2)References (4)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody (ab6165)

    Key features and details

    • Rabbit polyclonal to beta Synuclein
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Primary
    Product image
    Anti-SOX9 antibody [EPR14335] (ab185230)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
    Product image
    Recombinant Human beta Synuclein protein (ab48853)

    View more associated products

    Overview

    • Product name

      Anti-beta Synuclein antibody
      See all beta Synuclein primary antibodies
    • Description

      Rabbit polyclonal to beta Synuclein
    • Host species

      Rabbit
    • Specificity

      Less than 0.1% cross reactivity against human alpha-synuclein Cross reactivity: This antibody is known to react with human and rat. Not yet tested in other species.
    • Tested Applications & Species

      Application Species
      IHC-P
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide (IEPLMEPEGSYEDPPQE) corresponding to the carboxyl terminal of human beta-synuclein (108- 125) and was conjugated to diphtheria toxoid via a cystine residue added to the carboxyl terminus of the peptide. The conjugate was cross-linked by maleimidocaproyl-N-hydroxy succinimide.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7
    • Concentration information loading...
    • Purity

      Whole antiserum
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Parkinson's disease
      • Synuclein
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Immunizing Peptide (Blocking)

      • Recombinant Human beta Synuclein protein (ab48853)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human beta Synuclein protein (ab48853)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6165 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-P
    Human
    Application Abreviews Notes
    IHC-P (1)
    1/500 - 1/2000.
    Notes
    IHC-P
    1/500 - 1/2000.

    Target

    • Function

      Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease.
    • Tissue specificity

      Expressed predominantly in brain; concentrated in presynaptic nerve terminals.
    • Sequence similarities

      Belongs to the synuclein family.
    • Post-translational
      modifications

      Phosphorylated. Phosphorylation by G-protein coupled receptor kinases (GRK) is more efficient than phosphorylation by CK1, CK2 and CaM-kinase II.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession Q16143 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6620 Human
      • Omim: 602569 Human
      • SwissProt: Q16143 Human
      • Unigene: 90297 Human
      • Alternative names

        • 14 kDa brain-specific protein antibody
        • Beta-synuclein antibody
        • Phosphoneuroprotein 14 antibody
        • PNP14 antibody
        • Sncb antibody
        • Synuclein beta antibody
        • SYUB_HUMAN antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody (ab6165)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody (ab6165)
        ab6165 staining beta synuclein in human occipital cortex.
        Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
        Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

      Protocols

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (4)

      Publishing research using ab6165? Please let us know so that we can cite the reference in this datasheet.

      ab6165 has been referenced in 4 publications.

      • Lahut S  et al. Blood RNA biomarkers in prodromal PARK4 and rapid eye movement sleep behavior disorder show role of complexin 1 loss for risk of Parkinson's disease. Dis Model Mech 10:619-631 (2017). PubMed: 28108469
      • Helwig M  et al. Brain propagation of transduced a-synuclein involves non-fibrillar protein species and is enhanced in a-synuclein null mice. Brain 139:856-70 (2016). WB ; Mouse . PubMed: 26719384
      • Beyer K  et al. The decrease of {beta}-synuclein in cortical brain areas defines a molecular subgroup of dementia with Lewy bodies. Brain : (2010). WB ; Human . PubMed: 20959308
      • Iwata A  et al. alpha-Synuclein affects the MAPK pathway and accelerates cell death. J Biol Chem 276:45320-9 (2001). WB ; Human . PubMed: 11560921

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-3 of 3 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta Synuclein antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rat Tissue sections (Brain, Cortex)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citric Acid
      Permeabilization
      No
      Specification
      Brain, Cortex
      Blocking step
      BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted May 23 2018

      Question

      Can you please advise me on which blocking peptide to use against rabbit anti-beta synuclein (ab6165). We are using it in human tissue for immunohistochemistry.

      Please let me know if you require any further information.

      Looking forward for your response. When replying back can you please address the email “Dear Juli” as all replies come through a centralised email. This will avoid any confusion our end.

      Thanks

      Kind regards,

      Read More

      Abcam community

      Verified customer

      Asked on Oct 15 2012

      Answer

      Thank you for your enquiry regarding ab6165.

      I regret to inform you that the blocking peptide is not available commercially.

      Apologies!

      Read More

      Abcam Scientific Support

      Answered on Oct 15 2012

      Question

      Please see customers request below:

      Can you please advise me on which blocking peptide to use against rabbit anti-beta synuclein (ab6165). We are using it in human tissue for immunohistochemistry.

      Please let me know if you require any further information.

      Thanks

      Kind regards,

      Read More

      Abcam community

      Verified customer

      Asked on Oct 12 2012

      Answer

      Thank you for your email.

      I have contacted our laboratory and inquired about this.

      I will get back to you with the updates soon.

      Read More

      Abcam Scientific Support

      Answered on Oct 12 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.