Key features and details
- Rabbit polyclonal to beta Synuclein - C-terminal
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-beta Synuclein antibody - C-terminal
See all beta Synuclein primary antibodies
DescriptionRabbit polyclonal to beta Synuclein - C-terminal
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
- Human brain (cortex) tissue. A549 cells and HeLa cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol
PBS is without Mg2+, Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab189276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/500.|
|WB||1/500 - 1/1000. Predicted molecular weight: 14 kDa.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionNon-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease.
Tissue specificityExpressed predominantly in brain; concentrated in presynaptic nerve terminals.
Sequence similaritiesBelongs to the synuclein family.
modificationsPhosphorylated. Phosphorylation by G-protein coupled receptor kinases (GRK) is more efficient than phosphorylation by CK1, CK2 and CaM-kinase II.
- Information by UniProt
- 14 kDa brain-specific protein antibody
- Beta-synuclein antibody
- Phosphoneuroprotein 14 antibody
All lanes : Anti-beta Synuclein antibody - C-terminal (ab189276) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate with synthesized peptide
Predicted band size: 14 kDa
Immunohistochemistry of formalin-fixed, paraffin-embedded Human brain cortex tissue labeling beta Synuclein with ab189276 at 10 µg/ml.
Immunofluorescent analysis of A549 cells beta Synuclein with ab189276 at 1/100 dilution (left). Right panel is treated with the immunizing peptide.
ab189276 has been referenced in 1 publication.
- Gasperini L et al. The hnRNP raly regulates PRMT1 expression and interacts with the ALS-linked protein FUS: implication for reciprocal cellular localization. Mol Biol Cell N/A:mbcE18020108 (2018). PubMed: 30354839