Product nameAnti-beta Synuclein antibody [EP1537Y]
See all beta Synuclein primary antibodies
DescriptionRabbit monoclonal [EP1537Y] to beta Synuclein
Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human beta Synuclein aa 50-150. The exact sequence is proprietary.
Database link: Q16143
- Fetal brain lysate; human brain tissue; PC12 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab76111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/5000 - 1/20000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionNon-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease.
Tissue specificityExpressed predominantly in brain; concentrated in presynaptic nerve terminals.
Sequence similaritiesBelongs to the synuclein family.
modificationsPhosphorylated. Phosphorylation by G-protein coupled receptor kinases (GRK) is more efficient than phosphorylation by CK1, CK2 and CaM-kinase II.
- Information by UniProt
- 14 kDa brain-specific protein antibody
- Beta-synuclein antibody
- Phosphoneuroprotein 14 antibody
Anti-beta Synuclein antibody [EP1537Y] (ab76111) at 1/10000 dilution + fetal brain lysate at 10 µg
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 14 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Additional bands at: 21 kDa. We are unsure as to the identity of these extra bands.
ab76111 at 1/100-1/250 dilution staining beta Synuclein in PC12 cells by Immunofluorescence.
ab76111 at 1/100-1/250 dilution staining beta Synuclein in human brain by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This product has been referenced in:
- Benskey MJ et al. Silencing Alpha Synuclein in Mature Nigral Neurons Results in Rapid Neuroinflammation and Subsequent Toxicity. Front Mol Neurosci 11:36 (2018). Read more (PubMed: 29497361) »
- Popova B et al. Sumoylation Protects Against ß-Synuclein Toxicity in Yeast. Front Mol Neurosci 11:94 (2018). Read more (PubMed: 29636661) »