Recombinant
RabMAb

Anti-beta Synuclein antibody [EP1537Y] (ab76111)

Overview

  • Product name
    Anti-beta Synuclein antibody [EP1537Y]
    See all beta Synuclein primary antibodies
  • Description
    Rabbit monoclonal [EP1537Y] to beta Synuclein
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human beta Synuclein aa 50-150. The exact sequence is proprietary.
    Database link: Q16143

  • Positive control
    • Fetal brain lysate; human brain tissue; PC12 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab76111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/5000 - 1/20000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).
IP 1/40.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease.
    • Tissue specificity
      Expressed predominantly in brain; concentrated in presynaptic nerve terminals.
    • Sequence similarities
      Belongs to the synuclein family.
    • Post-translational
      modifications
      Phosphorylated. Phosphorylation by G-protein coupled receptor kinases (GRK) is more efficient than phosphorylation by CK1, CK2 and CaM-kinase II.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 14 kDa brain-specific protein antibody
      • Beta-synuclein antibody
      • Phosphoneuroprotein 14 antibody
      • PNP14 antibody
      • Sncb antibody
      • Synuclein beta antibody
      • SYUB_HUMAN antibody
      see all

    Images

    • ab76111 at 1/100-1/250 dilution staining beta Synuclein in PC12 cells by Immunofluorescence.
    • ab76111 at 1/100-1/250 dilution staining beta Synuclein in human brain by Immunohistochemistry, Paraffin-embedded tissue.
    • Anti-beta Synuclein antibody [EP1537Y] (ab76111) at 1/10000 dilution + fetal brain lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 14 kDa
      Observed band size: 18 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 21 kDa. We are unsure as to the identity of these extra bands.

    References

    This product has been referenced in:
    • Benskey MJ  et al. Silencing Alpha Synuclein in Mature Nigral Neurons Results in Rapid Neuroinflammation and Subsequent Toxicity. Front Mol Neurosci 11:36 (2018). Read more (PubMed: 29497361) »
    • Popova B  et al. Sumoylation Protects Against ß-Synuclein Toxicity in Yeast. Front Mol Neurosci 11:94 (2018). Read more (PubMed: 29636661) »

    See all 5 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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