Overview

  • Product name

    Anti-beta Synuclein antibody [EP1537Y] (HRP)
    See all beta Synuclein primary antibodies
  • Description

    Rabbit monoclonal [EP1537Y] to beta Synuclein (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human beta Synuclein aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: Q16143

  • Positive control

    • Mouse brain, Human brain
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab215783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).

Target

  • Function

    Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease.
  • Tissue specificity

    Expressed predominantly in brain; concentrated in presynaptic nerve terminals.
  • Sequence similarities

    Belongs to the synuclein family.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation by G-protein coupled receptor kinases (GRK) is more efficient than phosphorylation by CK1, CK2 and CaM-kinase II.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 14 kDa brain-specific protein antibody
    • Beta-synuclein antibody
    • Phosphoneuroprotein 14 antibody
    • PNP14 antibody
    • Sncb antibody
    • Synuclein beta antibody
    • SYUB_HUMAN antibody
    see all

Images

  • All lanes : Anti-beta Synuclein antibody [EP1537Y] (HRP) (ab215783) at 1/5000 dilution

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Human) Tissue Lysate - adult normal tissue

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 14 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?


    Exposure time: 4 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab215783 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab215783 has not yet been referenced specifically in any publications.

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