Overview

  • Product name
    Anti-Beta TRCP antibody [4C5D8]
    See all Beta TRCP primary antibodies
  • Description
    Mouse monoclonal [4C5D8] to Beta TRCP
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Beta TRCP aa 24-151.
    Database link: Q9Y297

  • Positive control
    • WB: beta TCRP (aa 24-151)-hIgGFc transfected HEK-293 cell lysate; Ramos, MCF7 and K562 cell lysates; Human beta TCRP (aa 24-151) recombinant protein. Flow Cyt: HeLa cells. IHC-P: Human esophageal cancer and rectum cancer tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab233638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 68 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

Target

  • Function
    Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22'. SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1/nuclear factor NF-kappa-B p105 subunit, ATF4, SMAD3, SMAD4, CDC25A, DLG1, FBXO5 and probably NFKB2. SCF(BTRC) mediates the ubiquitination of phosphorylated SNAI1. May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase. Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF. Required for proteolytic processing of GLI3.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 F-box domain.
    Contains 7 WD repeats.
  • Domain
    The N-terminal D domain mediates homodimerization.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta transducin repeat containing E3 ubiquitin protein ligase antibody
    • beta TrCP1 antibody
    • beta-transducin repeat containing antibody
    • BETA-TRCP antibody
    • betaTrCP antibody
    • btrC antibody
    • bTrCP antibody
    • bTrCP1 antibody
    • E3RSIkappaB antibody
    • F box and WD repeat protein 1B antibody
    • F box and WD repeats protein beta TrCP antibody
    • F-box and WD repeats protein beta-TrCP antibody
    • F-box/WD repeat protein 1A antibody
    • F-box/WD repeat-containing protein 1A antibody
    • FBW1A antibody
    • FBW1A_HUMAN antibody
    • FBXW1 antibody
    • FBXW1A antibody
    • FWD1 antibody
    • HOS antibody
    • mKIAA4123 antibody
    • pIkappaBalpha-E3 receptor subunit antibody
    • Slimb antibody
    see all

Images

  • Anti-Beta TRCP antibody [4C5D8] (ab233638) at 1/500 dilution + Human beta TCRP (aa 24-151) recombinant protein

    Predicted band size: 68 kDa



    Expected MW is 40.2 kDa.

  • All lanes : Anti-Beta TRCP antibody [4C5D8] (ab233638) at 1/500 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : beta TCRP (aa 24-151)-hIgGFc transfected HEK-293 cell lysate

    Predicted band size: 68 kDa

  • All lanes : Anti-Beta TRCP antibody [4C5D8] (ab233638) at 1/500 dilution

    Lane 1 : Ramos (human Burkitt's lymphoma cell line) cell lysate
    Lane 2 : NCF7 (human breast adenocarcinoma cell line) cell lysate
    Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate

    Predicted band size: 68 kDa

  • Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Beta TRCP with ab233638 at 1/200 dilution (green) compared with a negative control (red).

  • Paraffin-embedded human esophageal cancer tissue stained for Beta TRCP using ab233638 at 1/200 dilution in immunohistochemical analysis.

  • Paraffin-embedded human rectum cancer tissue stained for Beta TRCP using ab233638 at 1/200 dilution in immunohistochemical analysis.

References

ab233638 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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