• Product name
  • Description
    Goat polyclonal to Beta TRCP
  • Host species
  • Specificity
    This antibody is expected to recognise both reported isoforms (NP_378663.1; NP_003930.1).
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Recombinant fragment corresponding to Human Beta TRCP aa 2-14 (Cysteine residue).


    Database link: Q9Y297

  • Positive control
    • WB: HeLa cell lysate. IHC-P: Human heart tissue.



Our Abpromise guarantee covers the use of ab223233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 65 kDa.
IHC-P Use a concentration of 7.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22'. SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1/nuclear factor NF-kappa-B p105 subunit, ATF4, SMAD3, SMAD4, CDC25A, DLG1, FBXO5 and probably NFKB2. SCF(BTRC) mediates the ubiquitination of phosphorylated SNAI1. May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase. Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF. Required for proteolytic processing of GLI3.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 F-box domain.
    Contains 7 WD repeats.
  • Domain
    The N-terminal D domain mediates homodimerization.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta transducin repeat containing E3 ubiquitin protein ligase antibody
    • beta TrCP1 antibody
    • beta-transducin repeat containing antibody
    • BETA-TRCP antibody
    • betaTrCP antibody
    • btrC antibody
    • bTrCP antibody
    • bTrCP1 antibody
    • E3RSIkappaB antibody
    • F box and WD repeat protein 1B antibody
    • F box and WD repeats protein beta TrCP antibody
    • F-box and WD repeats protein beta-TrCP antibody
    • F-box/WD repeat protein 1A antibody
    • F-box/WD repeat-containing protein 1A antibody
    • FBW1A antibody
    • FBW1A_HUMAN antibody
    • FBXW1 antibody
    • FBXW1A antibody
    • FWD1 antibody
    • HOS antibody
    • mKIAA4123 antibody
    • pIkappaBalpha-E3 receptor subunit antibody
    • Slimb antibody
    see all


  • Anti-Beta TRCP antibody (ab223233) at 1 µg/ml + HeLa cell lysate in RIPA buffer at 35 µg

    Predicted band size: 65 kDa
    Observed band size: 65 kDa

    Primary incubation was 1 hour. Detected using chemiluminescence.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling Deta TRCP with ab223233 at 7.5 µg/ml. Heat induced antigen retrieval was performed with citrate buffer pH 6. HRP-staining.

  • Negative control Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue without primary antibody.


ab223233 has not yet been referenced specifically in any publications.

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