Overview

  • Product name

    Anti-beta TRCP2/HOS antibody
    See all beta TRCP2/HOS primary antibodies
  • Description

    Rabbit polyclonal to beta TRCP2/HOS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Chicken
  • Immunogen

    Recombinant fragment corresponding to Human beta TRCP2/HOS aa 32-321 (internal sequence).
    Database link: Q9UKB1

  • Positive control

    • 293T, A431, H1299 and HeLaS3 cell lysates This antibody gave a positive result when used in the following formaldehyde fixed cell lines: U87-MG
  • General notes

    Protein previously labeled as beta TRCP2.

Properties

Applications

Our Abpromise guarantee covers the use of ab137770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 62 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. SCF(FBXW11) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(FBXW11) mediates the ubiquitination of phosphorylated NFKBIA, which degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. SCF(FBXW11) mediates the ubiquitination of IFNAR1. Targets phosphorylation-dependent degradation of beta-catenin. Involved in the oxidative stress-induced a ubiquitin-mediated decrease in RCAN1. Mediates the degradation of CDC25A induced by ionizing radiation in cells progressing through S phase and thus may function in the intra-S-phase checkpoint. Has an essential role in the control of the clock-dependent transcription via degradation of PER1 and phosphorylated PER2. Is target of human immunodeficiency virus type 1 (HIV-1) protein VPU to polyubiquitinate and deplete BST2 from cells and antagonize its antiviral action.
  • Sequence similarities

    Contains 1 F-box domain.
    Contains 7 WD repeats.
  • Domain

    The N-terminal D domain mediates homodimerization.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • beta Transducin Repeat Containing Protein 2 antibody
    • BTRC2 antibody
    • BTRCP2 antibody
    • F-box and WD repeats protein beta-TrCP2 antibody
    • F-box and WD-40 Domain Protein 1B antibody
    • F-box Protein FBW1B antibody
    • F-box/WD repeat-containing protein 11 antibody
    • F-box/WD repeat-containing protein 1B antibody
    • FBW1B antibody
    • FBW1B_HUMAN antibody
    • FBXW11 antibody
    • FBXW1B antibody
    • Homologous to Slimb protein antibody
    • HOS antibody
    see all

Images

  • ICC/IF image of ab137770 stained U87-MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab137770 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-beta TRCP2/HOS antibody (ab137770) at 1/500 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 62 kDa



    7.5% SDS PAGE

References

This product has been referenced in:

  • Huang Y  et al. S6K1 phosphorylation-dependent degradation of Mxi1 by ß-Trcp ubiquitin ligase promotes Myc activation and radioresistance in lung cancer. Theranostics 8:1286-1300 (2018). Read more (PubMed: 29507620) »
See 1 Publication for this product

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