• Product name
    Anti-Beta TRCP/HOS antibody
    See all Beta TRCP/HOS primary antibodies
  • Description
    Rabbit polyclonal to Beta TRCP/HOS
  • Host species
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Beta TRCP/HOS aa 550 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab93745)

  • Positive control
    • This antibody gave a positive signal in Caco2 whole cell lysate. This antibody gave a positive signal in the following cell types: T47D. It also gave a positive signal in FFPE human testis tissue sections.



Our Abpromise guarantee covers the use of ab71753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 68 kDa).
ICC/IF Use a concentration of 5 µg/ml.


  • Function
    Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22'. SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1/nuclear factor NF-kappa-B p105 subunit, ATF4, SMAD3, SMAD4, CDC25A, DLG1, FBXO5 and probably NFKB2. SCF(BTRC) mediates the ubiquitination of phosphorylated SNAI1. May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase. Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF. Required for proteolytic processing of GLI3.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 F-box domain.
    Contains 7 WD repeats.
  • Domain
    The N-terminal D domain mediates homodimerization.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta transducin repeat containing E3 ubiquitin protein ligase antibody
    • beta TrCP1 antibody
    • beta-transducin repeat containing antibody
    • BETA-TRCP antibody
    • betaTrCP antibody
    • btrC antibody
    • bTrCP antibody
    • bTrCP1 antibody
    • E3RSIkappaB antibody
    • F box and WD repeat protein 1B antibody
    • F box and WD repeats protein beta TrCP antibody
    • F-box and WD repeats protein beta-TrCP antibody
    • F-box/WD repeat protein 1A antibody
    • F-box/WD repeat-containing protein 1A antibody
    • FBW1A antibody
    • FBW1A_HUMAN antibody
    • FBXW1 antibody
    • FBXW1A antibody
    • FWD1 antibody
    • HOS antibody
    • mKIAA4123 antibody
    • pIkappaBalpha-E3 receptor subunit antibody
    • Slimb antibody
    see all


  • Anti-Beta TRCP/HOS antibody (ab71753) at 1 µg/ml + Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 68 kDa
    Observed band size: 62 kDa
    why is the actual band size different from the predicted?

    Exposure time: 20 minutes

    The 62 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Human F-box/WD repeat-containing protein 1A (Beta TRCP/HOS)

  • ICC/IF image of ab71753 stained T47D cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab71753 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of Beta TRCP/HOS staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab71753, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:
  • Liang J  et al. ß-transducin repeat-containing E3 ubiquitin protein ligase inhibits migration, invasion and proliferation of glioma cells. Oncol Lett 14:3131-3135 (2017). Read more (PubMed: 28928851) »
  • Liang J  et al. Expression of ß-transducin repeat-containing E3 ubiquitin protein ligase in human glioma and its correlation with prognosis. Oncol Lett 9:2651-2656 (2015). Read more (PubMed: 26137122) »
See all 2 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Human Cultured Cells (HeLa cell)
Yes - 0.1% PBST, RT 15min
HeLa cell
Blocking step
CAS-Block (Invitrogen) as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

Ms. Yeongmi Cheon

Verified customer

Submitted Aug 07 2017

Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Loading amount
20000 cells
Gel Running Conditions
Reduced Denaturing (gel 10%)
Mouse Cell lysate - whole cell (Mouse Embryonic Stem Cells)
Mouse Embryonic Stem Cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C

Joe Segal

Verified customer

Submitted Jul 17 2013


Thank you for contacting us and your interest in our products.
The Anti-Beta TRCP antibody (ab71753) has as yet not been tested with mouse samples. This is therefore information we would be interested in for the testing discount scheme. The discount code requested is as follows:

Discount code: xxxxxx
Expiration date: 28th of November 2012
The terms and conditions of the testing discount scheme can be found from the following link:
I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

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Thank you for your note.  This is to let you know that I have just contacted and asked our Account Department to raise a credit note for you - for the cost of one vial of ab71753. For your information, the internal reference note for this credit is CNXXXXXX. You can use this credit in the near future for any of the products which are in the catalogue. I hope this helps and if I can assist further, please do not hesitate to contact me.

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Thank you for getting back to me. I can offer you either a new vial as a free of charge replacement or a credit note which can be used for future purchase. Please discuss it with your customer and let me know how you wish to proceed with this complaint. I look forward to hearing from you soon.      

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Thank you for your enquiry regarding ab71753 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.   - Has the customer applied any loading control?   - Does the detection system work fine? Have you used it successfully with another primary antibody?   Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.        

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