Key features and details
- Mouse monoclonal [OTI1D4] to Beta TRCP/HOS
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Beta TRCP/HOS antibody [OTI1D4]
See all Beta TRCP/HOS primary antibodies
DescriptionMouse monoclonal [OTI1D4] to Beta TRCP/HOS
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Beta TRCP/HOS aa 52-354. Protein produced in E.coli. NP_378663
Database link: Q9Y297
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY Beta TRCP/HOS cDNA.
The clone number has been updated from 1D4 to OTI1D4, both clone numbers name the same clone.
This product was previously labelled as Beta TRCP
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol, PBS
Concentration information loading...
Purification notesPurified from cell culture supernatant by affinity chromatography
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- SCF Complex E3 Ligase
Our Abpromise guarantee covers the use of ab118006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Predicted molecular weight: 69 kDa.|
FunctionSubstrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22'. SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1/nuclear factor NF-kappa-B p105 subunit, ATF4, SMAD3, SMAD4, CDC25A, DLG1, FBXO5 and probably NFKB2. SCF(BTRC) mediates the ubiquitination of phosphorylated SNAI1. May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase. Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF. Required for proteolytic processing of GLI3.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 F-box domain.
Contains 7 WD repeats.
DomainThe N-terminal D domain mediates homodimerization.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Beta transducin repeat containing E3 ubiquitin protein ligase antibody
- beta TrCP1 antibody
- beta-transducin repeat containing antibody
All lanes : Anti-Beta TRCP/HOS antibody [OTI1D4] (ab118006) at 1/500 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T lysate transfected with pCMV6-ENTRY Beta TRCP/HOS cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 69 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab118006 has been referenced in 2 publications.