Overview

  • Product name

    Anti-Beta TRCP/HOS antibody [OTI1D4]
    See all Beta TRCP/HOS primary antibodies
  • Description

    Mouse monoclonal [OTI1D4] to Beta TRCP/HOS
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Beta TRCP/HOS aa 52-354. Protein produced in E.coli. NP_378663
    Database link: Q9Y297

  • Positive control

    • WB: HEK-293T cell lysate transfected with pCMV6-ENTRY Beta TRCP/HOS cDNA.
  • General notes

    The clone number has been updated from 1D4 to OTI1D4, both clone numbers name the same clone.

     This product was previously labelled as Beta TRCP

     

Properties

Applications

Our Abpromise guarantee covers the use of ab118006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 69 kDa.

Target

  • Function

    Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22'. SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1/nuclear factor NF-kappa-B p105 subunit, ATF4, SMAD3, SMAD4, CDC25A, DLG1, FBXO5 and probably NFKB2. SCF(BTRC) mediates the ubiquitination of phosphorylated SNAI1. May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase. Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF. Required for proteolytic processing of GLI3.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 F-box domain.
    Contains 7 WD repeats.
  • Domain

    The N-terminal D domain mediates homodimerization.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta transducin repeat containing E3 ubiquitin protein ligase antibody
    • beta TrCP1 antibody
    • beta-transducin repeat containing antibody
    • BETA-TRCP antibody
    • betaTrCP antibody
    • btrC antibody
    • bTrCP antibody
    • bTrCP1 antibody
    • E3RSIkappaB antibody
    • F box and WD repeat protein 1B antibody
    • F box and WD repeats protein beta TrCP antibody
    • F-box and WD repeats protein beta-TrCP antibody
    • F-box/WD repeat protein 1A antibody
    • F-box/WD repeat-containing protein 1A antibody
    • FBW1A antibody
    • FBW1A_HUMAN antibody
    • FBXW1 antibody
    • FBXW1A antibody
    • FWD1 antibody
    • HOS antibody
    • mKIAA4123 antibody
    • pIkappaBalpha-E3 receptor subunit antibody
    • Slimb antibody
    see all

Images

  • All lanes : Anti-Beta TRCP/HOS antibody [OTI1D4] (ab118006) at 1/500 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK-293T lysate transfected with pCMV6-ENTRY Beta TRCP/HOS cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 69 kDa

References

This product has been referenced in:

See 1 Publication for this product

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