Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205)
Key features and details
- Mouse monoclonal [1E1-E8-H4] to beta Tubulin - Microtubule Marker
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG1
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Overview
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Product name
Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker
See all beta Tubulin primary antibodies -
Description
Mouse monoclonal [1E1-E8-H4] to beta Tubulin - Microtubule Marker -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, African green monkey -
Immunogen
Recombinant fragment corresponding to Human beta Tubulin.
Database link: P07437 -
Positive control
- A431, HeLa and Jurkat cell lysates.
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General notes
This product was changed from ascites to tissue culture supernatant on 17.08.2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituent: 50% Glycerol -
Concentration information loading...
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Purity
Concentrated Culture Supernatant -
Clonality
Monoclonal -
Clone number
1E1-E8-H4 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (3) |
1/300.
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WB | (3) |
1/5000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
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Notes |
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ICC/IF
1/300. |
WB
1/5000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). |
Target
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Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
Tissue specificity
Ubiquitously expressed with highest levels in spleen, thymus and immature brain. -
Involvement in disease
Cortical dysplasia, complex, with other brain malformations 6
Skin creases, congenital symmetric circumferential, 1 -
Sequence similarities
Belongs to the tubulin family. -
Domain
The highly acidic C-terminal region may bind cations such as calcium. -
Post-translational
modificationsSome glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. -
Cellular localization
Cytoplasm, cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 203068 Human
- Entrez Gene: 22154 Mouse
- Entrez Gene: 29214 Rat
- Omim: 191130 Human
- SwissProt: P07437 Human
- SwissProt: P99024 Mouse
- SwissProt: P69897 Rat
- Unigene: 636480 Human
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Alternative names
- Beta 4 tubulin antibody
- Beta 5 tubulin antibody
- beta Ib tubulin antibody
see all
Images
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All lanes : Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205) at 1/5000 dilution
Lane 1 : C6 Cell lysate
Lane 2 : K562 Cell lysate
Lane 3 : COS7 Cell lysate
Lane 4 : 3T3 Cell lysate
Lane 5 : HeLa Cell lysate
Predicted band size: 49 kDa -
All lanes : Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205) at 1/500 dilution
Lane 1 : COS7 cell lysate
Lane 2 : LNCap cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : C2C12 cell lysate
Predicted band size: 49 kDaThis image was produced with antibody produced in ascites.
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Immunocytochemistry stain of Hela using ab131205(1:300). This image was produced with antibody produced in ascites.
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ab131205 stability test under 37ºC for 50 hours compared to 4°C for 50 hours. This image was produced with antibody produced in ascites.
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All lanes : Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Predicted band size: 49 kDa
Observed band size: 49 kDaThis image was produced with antibody produced in ascites.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab131205 has been referenced in 15 publications.
- Ward EN et al. Machine learning assisted interferometric structured illumination microscopy for dynamic biological imaging. Nat Commun 13:7836 (2022). PubMed: 36543776
- La Padula PH et al. Acute hypobaric hypoxia and cardiac energetic response in prepubertal rats: Role of nitric oxide. Exp Physiol 106:1235-1248 (2021). PubMed: 33724589
- Vanasco V et al. Mitochondrial Dynamics and VMP1-Related Selective Mitophagy in Experimental Acute Pancreatitis. Front Cell Dev Biol 9:640094 (2021). PubMed: 33816487
- Lu M et al. The structure and global distribution of the endoplasmic reticulum network are actively regulated by lysosomes. Sci Adv 6:N/A (2020). PubMed: 33328230
- Grigoruta M et al. Psychological Stress Phenocopies Brain Mitochondrial Dysfunction and Motor Deficits as Observed in a Parkinsonian Rat Model. Mol Neurobiol 57:1781-1798 (2020). PubMed: 31836946