Key features and details
- Mouse monoclonal [AA2] to beta Tubulin (HRP)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
- Isotype: IgG1
Product nameAnti-beta Tubulin antibody [AA2] (HRP)
See all beta Tubulin primary antibodies
DescriptionMouse monoclonal [AA2] to beta Tubulin (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Cow beta Tubulin. (Bovine brain tubulin).
EpitopeRecognizes amino acids 416-431
- WB: HeLa, NIH/3T3, PC-12 and HepG2 cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityImmunogen affinity purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab235761 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Tissue specificityUbiquitously expressed with highest levels in spleen, thymus and immature brain.
Involvement in diseaseCortical dysplasia, complex, with other brain malformations 6
Skin creases, congenital symmetric circumferential, 1
Sequence similaritiesBelongs to the tubulin family.
DomainThe highly acidic C-terminal region may bind cations such as calcium.
modificationsSome glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
Cellular localizationCytoplasm, cytoskeleton.
- Information by UniProt
- Beta 4 tubulin antibody
- Beta 5 tubulin antibody
- beta Ib tubulin antibody
This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 50 mins before being transferred onto a nitrocellulose membrane at 30V for 70 mins. The membrane was then blocked for an hour using 3% non-fat milk before being incubated with ab235761 overnight at 4°C. Antibody binding was visualized using ECL development solution ab133406.
Samples: 1. HeLa (20µg) 2. NIH 3T3 (20µg) 3. PC12 (20µg) 4. HepG2 (20µg).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235761 has not yet been referenced specifically in any publications.