Product nameAnti-beta Tubulin antibody
See all beta Tubulin primary antibodies
DescriptionGoat polyclonal to beta Tubulin
Specificityab21057 detects a band of ~50kDa in Hela and A431 lysates (data for A431 not shown). These bands are blocked by the addition of the immunizing peptide, ab20775.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human, African green monkey
Predicted to work with: Rat, Chicken
- HeLa whole cell lysate, HeLa nuclear lysate and A431 cell lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: 1% BSA
This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab21057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human beta Tubulin peptide (ab20775).|
FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Tissue specificityUbiquitously expressed with highest levels in spleen, thymus and immature brain.
Involvement in diseaseCortical dysplasia, complex, with other brain malformations 6
Skin creases, congenital symmetric circumferential, 1
Sequence similaritiesBelongs to the tubulin family.
DomainThe highly acidic C-terminal region may bind cations such as calcium.
modificationsSome glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
Cellular localizationCytoplasm, cytoskeleton.
- Information by UniProt
- Beta 4 tubulin antibody
- Beta 5 tubulin antibody
- beta Ib tubulin antibody
All lanes : Anti-beta Tubulin antibody (ab21057) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa whole cell lysate with
Human beta Tubulin peptide (ab20775) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
All lanes : Rabbit anti Goat polyclonal at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
ab21057 detects a band of ~ 50kDa in Hela whole cell lysate, HeLa nuclear lysate and A431 lysates (data for HeLa nuclear and A431 not shown). These bands are blocked by the addition of the immunizing peptide, ab20775.
Ab21057 staining beta-tubulin in asynchronous HeLa cells (green). Cells were counterstained with DAPI (red) to show the nucleus. Secondary antibody: Donkey anti-Goat antibody conjugated to Alexafluor 488 ® (Please refer to the Abreview for further details).
Western blot image using the Optiblot Reducing Electrophoresis Kit - 10 x 10 cm (4-20%) (ab119220) with the
Prism Ultra Protein Ladder (ab116028) 5µl used. We recommend using our ECL substrate kit (ab65623) .
20ug of Lysate per lane and detection using ab21057 diluted to 1ug/ml.
Lane 1: Hela cell lysate.
Lane 2: Jurkat cell lysate
Lane 3: A431 cell lysate
Lane 4: HEK293 cell lysate
Lane 5: HepG2 cell lysate.
ab21057 staining beta tubulin in CV1 (fibroblasts) cells from African green monkey by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and permeabilized with methanol. Samples were incubated with primary antibody (1/500 in PBS + 5% BSA) for 1 hour 30 minutes at 22°C. An Alexa Fluor® 488-conjugated Donkey anti-goat polyclonal was used as the secondary antibody (1/200).
This product has been referenced in:
- Armaka M et al. The p55TNFR-IKK2-Ripk3 axis orchestrates arthritis by regulating death and inflammatory pathways in synovial fibroblasts. Nat Commun 9:618 (2018). Read more (PubMed: 29434332) »
- Campion CG et al. COMMD5/HCaRG Hooks Endosomes on Cytoskeleton and Coordinates EGFR Trafficking. Cell Rep 24:670-684.e7 (2018). Read more (PubMed: 30021164) »