Product nameAnti-beta Tubulin antibody [EPR16774] (HRP)
See all beta Tubulin primary antibodies
DescriptionRabbit monoclonal [EPR16774] to beta Tubulin (HRP)
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Dog, Drosophila melanogaster, Monkey, Zebrafish, Xenopus tropicalis
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human beta Tubulin aa 400 to the C-terminus. The exact sequence is proprietary.
Database link: Q9BVA1
- WB: HeLa, NIH3T3 and PC12 whole cell lysates and mouse and rat brain tissue lysates. IHC-P: FFPE human cerebral cortex (normal) tissue sections.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.1% Proclin
Constituents: 1% BSA, PBS, 30% Glycerol
Concentration information loading...
PurityProtein A purified
- Anti-beta Tubulin antibody [EPR16774] (ab179513)
- Anti-beta Tubulin antibody [EPR16774] (Alexa Fluor® 488) (ab204685)
- Anti-beta Tubulin antibody [EPR16774] (Alexa Fluor® 647) (ab204686)
- Anti-beta Tubulin antibody [EPR16774] (Alexa Fluor® 594) (ab206369)
- Anti-beta Tubulin antibody [EPR16774] (Alexa Fluor® 405) (ab206370)
- Anti-beta Tubulin antibody [EPR16774] (Alexa Fluor® 555) (ab206627)
- Anti-beta Tubulin antibody [EPR16774] (Alexa Fluor® 568) (ab208144)
- Anti-beta Tubulin antibody [EPR16774] (Phycoerythrin) (ab208873)
- Anti-beta Tubulin antibody [EPR16774] - BSA and Azide free (ab232361)
Our Abpromise guarantee covers the use of ab205790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).|
|IHC-P||1/166. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Tissue specificityUbiquitously expressed with highest levels in spleen, thymus and immature brain.
Involvement in diseaseCortical dysplasia, complex, with other brain malformations 6
Skin creases, congenital symmetric circumferential, 1
Sequence similaritiesBelongs to the tubulin family.
DomainThe highly acidic C-terminal region may bind cations such as calcium.
modificationsSome glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
Cellular localizationCytoplasm, cytoskeleton.
- Information by UniProt
- Beta 4 tubulin antibody
- Beta 5 tubulin antibody
- beta Ib tubulin antibody
IHC image of beta Tubulin staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab205790, 1/166 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-beta Tubulin antibody [EPR16774] (HRP) (ab205790) at 1/5000 dilution
Lane 1 :
HeLa whole cell lysate (ab150035)
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 4 : Brain (Mouse) Tissue Lysate
Lane 5 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab205790 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab205790 has not yet been referenced specifically in any publications.