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  1. Link

    beta-tubulin-antibody-loading-control-ab6046.pdf

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Isotype/Loading Controls Loading Controls Tubulin
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Anti-beta Tubulin antibody - Loading Control (ab6046)

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  • SDS
Reviews (61)Q&A (18)References (955)

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Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody - Loading Control (ab6046)
  • Western blot - Anti-beta Tubulin antibody - Loading Control (ab6046)
  • Western blot - Anti-beta Tubulin antibody - Loading Control (ab6046)
  • Immunoprecipitation - Anti-beta Tubulin antibody - Loading Control (ab6046)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody - Loading Control (ab6046)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody - Loading Control (ab6046)

Key features and details

  • Rabbit polyclonal to beta Tubulin - Loading Control
  • Suitable for: WB, ICC/IF, IHC-P, IP
  • Reacts with: Mouse, Rat, Human, Chinese hamster
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-beta Tubulin antibody [EPR16774] (ab179513)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-beta Tubulin antibody - Loading Control
    See all beta Tubulin primary antibodies
  • Description

    Rabbit polyclonal to beta Tubulin - Loading Control
  • Host species

    Rabbit
  • Specificity

    This antibody detects a single clean band at 50kD representing beta Tubulin. This band is significantly reduced by using peptide blocking.
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Chinese hamster
    Predicted to work with: Chicken, Pig, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab20775)

  • Positive control

    • WB: HeLa, A431, MCF7, NIH3T3, PC12 CHO/K1, and 293 cell lysates; IP: HeLa whole cell extract; ICC/IF: HeLa cells; IHC-P: Human liver carcinoma tissue section.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Isotype/Loading Controls
    • Loading Controls
    • Tubulin
    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Isotype/Loading Controls
    • Loading Controls
    • Beta Tubulin
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • HRP Anti-beta Tubulin antibody - Loading Control (ab21058)
    • Anti-beta Tubulin antibody [AA2] (ab231082)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Immunizing Peptide (Blocking)

    • Human beta Tubulin peptide (ab20775)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human beta Tubulin protein (ab70187)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-SDHB antibody [21A11AE7] (ab14714)
    • Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
    • Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)
    • Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
    • Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (38)
1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ICC/IF (11)
Use a concentration of 1 µg/ml.
IHC-P (1)
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP (1)
Use at an assay dependent concentration.
Notes
WB
1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Tissue specificity

    Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
  • Involvement in disease

    Cortical dysplasia, complex, with other brain malformations 6
    Skin creases, congenital symmetric circumferential, 1
  • Sequence similarities

    Belongs to the tubulin family.
  • Domain

    The highly acidic C-terminal region may bind cations such as calcium.
  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
    Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
    Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
  • Cellular localization

    Cytoplasm, cytoskeleton.
  • Target information above from: UniProt accession P07437 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 396254 Chicken
    • Entrez Gene: 203068 Human
    • Entrez Gene: 22154 Mouse
    • Entrez Gene: 733686 Pig
    • Entrez Gene: 29214 Rat
    • Entrez Gene: 380418 Xenopus laevis
    • Entrez Gene: 386701 Zebrafish
    • Omim: 191130 Human
    • SwissProt: P07437 Human
    • SwissProt: P99024 Mouse
    • SwissProt: Q767L7 Pig
    • SwissProt: P69897 Rat
    • SwissProt: Q91575 Xenopus laevis
    • Unigene: 636480 Human
    • Unigene: 46552 Xenopus laevis
    see all
  • Alternative names

    • Beta 4 tubulin antibody
    • Beta 5 tubulin antibody
    • beta Ib tubulin antibody
    • Beta1 tubulin antibody
    • Class I beta tubulin antibody
    • M40 antibody
    • MGC117247 antibody
    • MGC16435 antibody
    • OK/SW cl.56 antibody
    • OK/SWcl.56 antibody
    • TBB5_HUMAN antibody
    • TUBB 1 antibody
    • TUBB 2 antibody
    • TUBB 5 antibody
    • TUBB antibody
    • TUBB1 antibody
    • TUBB2 antibody
    • TUBB5 antibody
    • tubulin beta 1 chain antibody
    • Tubulin beta 2 chain antibody
    • tubulin beta 5 chain antibody
    • Tubulin beta chain antibody
    • Tubulin beta class I antibody
    • tubulin beta polypeptide antibody
    • Tubulin beta-5 chain antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody - Loading Control (ab6046)
    Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody - Loading Control (ab6046)

    ab6046 staining beta Tubulin in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab6046 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 100% methanol (5 min).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Western blot - Anti-beta Tubulin antibody - Loading Control (ab6046)
    Western blot - Anti-beta Tubulin antibody - Loading Control (ab6046)
    All lanes : Anti-beta Tubulin antibody - Loading Control (ab6046) at 1/500 dilution

    Lane 1 : HeLa Cell lysate
    Lane 2 : A431 Cell lysate
    Lane 3 : MCF7 Cell lysate
    Lane 4 : 293 Cell lysate
    Lane 5 : HeLa Cell lysate with Human beta Tubulin peptide (ab20775) at 1 µg/ml
    Lane 6 : A431 Cell lysate with Human beta Tubulin peptide (ab20775) at 1 µg/ml
    Lane 7 : MCF7 Cell lysate with Human beta Tubulin peptide (ab20775) at 1 µg/ml
    Lane 8 : 293 Cell lysate with Human beta Tubulin peptide (ab20775) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds
  • Western blot - Anti-beta Tubulin antibody - Loading Control (ab6046)
    Western blot - Anti-beta Tubulin antibody - Loading Control (ab6046)
    All lanes : Anti-beta Tubulin antibody - Loading Control (ab6046) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : NIH3T3 (Mouse embryo fibroblast cell line) whole cell lysate
    Lane 3 : PC12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 4 : CHO/K1 (Chinese hamster ovary cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 51 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds
  • Immunoprecipitation - Anti-beta Tubulin antibody - Loading Control (ab6046)
    Immunoprecipitation - Anti-beta Tubulin antibody - Loading Control (ab6046)
    Beta Tubulin was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Tubulin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab6046.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 50kDa: beta Tubulin.
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody - Loading Control (ab6046)
    Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody - Loading Control (ab6046)

    ICC/IF image of ab6046 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab150081 Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody - Loading Control (ab6046)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody - Loading Control (ab6046)
    IHC image of beta Tubulin staining in human liver carcinoma FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6046, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (955)

Publishing research using ab6046? Please let us know so that we can cite the reference in this datasheet.

ab6046 has been referenced in 955 publications.

  • Velasco K  et al. Functional evaluation of 16 SCHAD missense variants: Only amino acid substitutions causing congenital hyperinsulinism of infancy lead to loss-of-function phenotypes in vitro. J Inherit Metab Dis 44:240-252 (2021). PubMed: 32876354
  • Zhang W  et al. The potential role of PSMA6 in modulating fat deposition in pigs by promoting preadipocyte proliferation and differentiation. Gene 769:145228 (2021). PubMed: 33096182
  • Li Z  et al. Potent aneugenicity of 1-methylpyrene in human cells dependent on metabolic activation by endogenous enzymes. Arch Toxicol 95:703-713 (2021). PubMed: 33057863
  • Clapper E  et al. The regulation of bcr-abl in hypoxia is through the mTOR pathway. Leuk Lymphoma 62:967-978 (2021). PubMed: 33251904
  • Giridharan VV  et al. Receptor for Advanced Glycation End Products (RAGE) Mediates Cognitive Impairment Triggered by Pneumococcal Meningitis. Neurotherapeutics 18:640-653 (2021). PubMed: 32886341
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-10 of 79 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-beta Tubulin antibody - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MDA-MB-231)
Permeabilization
No
Specification
MDA-MB-231
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Marsilius Mues

Verified customer

Submitted Mar 13 2021

Western blot abreview for Anti-beta Tubulin antibody - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293T, HepaRG, Huh7, HepG2)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
40 µg
Specification
293T, HepaRG, Huh7, HepG2
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Romain Parent

Verified customer

Submitted Oct 14 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-beta Tubulin antibody - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
African green monkey Cell (COS7 cells)
Permeabilization
Yes - Saponin
Specification
COS7 cells
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 05 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-beta Tubulin antibody - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (A549)
Permeabilization
Yes - Triton X-100
Specification
A549
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 16 2020

Western blot abreview for Anti-beta Tubulin antibody - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Fish Cell lysate - whole cell (ATCC-CRL-2872)
Gel Running Conditions
Reduced Non-Denaturing (Native) (10% SDS)
Loading amount
1e+006 cells
Specification
ATCC-CRL-2872
Blocking step
BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 10 2020

Western blot abreview for Anti-beta Tubulin antibody - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U-87MG cell line)
Gel Running Conditions
Reduced Denaturing (4-15% gradient polyacrylamide gel)
Loading amount
30 µg
Specification
U-87MG cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 05 2020

Western blot abreview for Anti-beta Tubulin antibody - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Multiple myeloma cancer cell line lysates)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
Multiple myeloma cancer cell line lysates
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 15 2020

Western blot abreview for Anti-beta Tubulin antibody - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (striatum)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
striatum
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Alexandre Magno

Verified customer

Submitted Jul 01 2019

Immunohistochemistry free floating abreview for Anti-beta Tubulin antibody - Loading Control

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (brain)
Specification
brain
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 12 2019

Immunocytochemistry abreview for Anti-beta Tubulin antibody - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (primary mouse astrocytes)
Permeabilization
Yes - 0.1% triton x100
Specification
primary mouse astrocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Qasim Alhadidi

Verified customer

Submitted Nov 27 2018

1-10 of 79 Abreviews or Q&A

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