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Dear Abcam People We are looking to do western blots of nuclear and cytoplasmic fractions of HeLa cells to investigate nuclear localization following UV stress. We need to use loading controls to show that the cytoplasmic fraction contains no nuclear component, and that the nuclear fraction contains no nuclear component. Which antibodies do you suggest we use for the nuclear fractions - beta tubulin or TATA binding protein? And for the cytoplasmic fraction? Could you give us catalogue numbers? Many thanks
Asked on Oct 20 2011
Thank you for contacting Abcam. I have searched our catalogue and found two antibodies which I believe will work for your experiments. The nuclear fraction loading control I would use is ab23474 which is a rabbit polyclonal to TATA binding protein. The other antibody I would recommend, for the cytoplasmic fraction is the ab8229, this is a goat polyclonal antibody to beta-actin. I hope that you find this information helpful. Please feel free to contact us again should you have further questions about these antibodies or any of the products in our catalogue.
Answered on Oct 20 2011