Question (37755) | Anti-beta Tubulin antibody - Loading Control (ab6046)

Go to datasheet (ab6046)

Question

Dear Abcam,



I purchased the anti-beta tubulin antibody (loading control, ab6046) and used it in western blotting.



I am using it to as a loading control for rat brain striatal tissue lysates.



After SDS Page, I cut the membrane at 40kDa and stained the top half (section 2 – see attached) using ab6046 (1:500 dilution) and the bottom half (section 3 – see attached with an anti-DARPP antibody that gives a band at 34 kDa).



The protocol was exactly the same for both halves of the membrane, the same secondary antibody was used (Biotin goat anti-rabbit IgG), the same number of washes etc but ab6046 gives high background. Also, there appears to be another band at 80 kDa. I used Invitrogen’s WesternDot kit and imaged the nitrocellulose membrane under UV illumination.

Please can you help?

Many thanks,

Answer

Thank you for your enquiry regarding ab6046 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.
As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.
- Could you please confirm the batch number and the Abcam Order number?
- How was the blocking carried out? Was BSA or milk or other agent used?
I look forward to hearing from you and hope to solve this problem as soon as possible.

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