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I can try using 5% BSA to see if that makes a difference - yes - please send me a vial from a different batch and I will give it a go. What BSA do you recommend I try?
Asked on Mar 01 2012
Thank you for your response.
This is to let you know that I have placed a new order for you - for one vial of ab6046 as a free of charge replacement (from a new batch: GR73104-1) and the new order number is 1044538.
I hope the second vial will work as it is expected, and please do let me know how you are getting on with this product.
I would suggest using 5% BSA in TBS-T as blocking agent and diluting the primary and the secondary antibody in this solution. My other advice would be to use (if it is possible) Nonidet-P40 (NP40) buffer which contains high concentration of detergent to make sure that the proteins are properly extracted from the brain (which contains high levels of lipids and phospholipids).
Nonidet-P40 (NP40) buffer:
150 mM sodium chloride
1.0% NP-40 (Triton X-100 can be substituted for NP-40)
50 mM Tris, pH 8.0
You can find some useful details at this site:
If you need any further assistance in the future, please do not hesitate to contact me.
Answered on Mar 01 2012