Question (40182) | Anti-beta Tubulin antibody - Loading Control (ab6046)

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Question

What antibody do you recommend as a loading controlfor:
nuclear fraction (HEK293T)?
cytoplasmic fraction(HEK293T)?
whole cell extract (HEK293T)?

Do you have one which works for each fraction?

Answer

Thank you for contacting us.

My recommendations are below. For a loading control for whole cell extracts, you could use any one of these, since the whole cell extract will contain all of these proteins. I have listed two proteins for the cytoplasmic fraction (GAPDH and tubulin). Both are appropriate but one may be preferable, depending on the size of your protein of interest.

nuclear fraction
Anti-TATA BP - ab818

cytoplasmic fraction
Anti-GAPDH - ab9485
Anti-tubulin - ab6046

whole cell extract
Anti-GAPDH - ab9485
Anti-tubulin - ab6046
Anti-TATBP - ab818

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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