Question (59612) | Anti-beta Tubulin antibody - Loading Control (ab6046)

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Today I ordered 100 ug of an anti- beta tubulin primary antibody. I received the antibody this afternoon (and I am grateful that I received it so quickly!). I briefly spun down the tube to get all of the antibody solution to the bottom of the tube. It should have contained 100 microliters in the tube. As I was putting aside aliquots to freeze at -20 degrees (in 10 microliter aliquots), the last tube had less than 5 microliters in it, instead of 10. Unfortunately, this precludes me from doing one western blot, which requires 1:500 dilution. Considering that the antibody cost over $300, this is approximately $30 that I spent and cannot use (10 microliters antibody is needed per western blot). I was wondering if its somehow possible to get another 10 microliters of the antibody so that I can complete the experiments which I had planned? This is a particularly important antibody for our lab, as it will serve as a loading control for all of our western blots. We often buy antibodies from Abcam and will plan on buying more in the future. However, at this point, it is not reasonable to buy another tube to get enough antibody for the 10th blot. Thank you, Jenny Linnoila, M.D., Ph.D. The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail.


Thank you for contacting us. I am sorry that the vial you received did not contain the full amount of the product and I apologize for the inconvenience. I have issued a free of charge replacement vial with the order number of xxxx. I have asked this to be shipped to you on Tuesday because of the holiday weekend.. Please do not hesitate to contact us if you need anything further.

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