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Western blot abreview for Anti-beta Tubulin antibody - Loading Control

Excellent
Abreviews
Go to datasheet (ab6046)
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Multiple myeloma cancer cell line lysates)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
Multiple myeloma cancer cell line lysates
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBS
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (HRP)

Detection

Detection method
ECL
Exposure
5 second(s)
Bands
Specific: ~55 kDa

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Submitted Jan 15 2020