Betulinic acid, TGR5 agonist (ab120654)
Key features and details
- Anticancer agent. TGR5 agonist.
- CAS Number: 472-15-1
- Soluble in DMSO to 50 mM (with warming)
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Betulinic acid, TGR5 agonist -
Description
Anticancer agent. TGR5 agonist. -
Biological description
Triterpenoid with various biological activities. Apoptosis inducer, with anticancer properties. Also shows anti-HIV, antimalarial and anti-inflammatory activity. TGR5 agonist (EC50 = 1.04 μM).
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CAS Number
472-15-1 -
Chemical structure
Properties
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Chemical name
(3β)-3-Hydroxylup-20(29)-en-28-oic acid -
Molecular weight
456.70 -
Molecular formula
C30H48O3 -
PubChem identifier
64971 -
Storage instructions
Store at +4°C. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 50 mM (with warming) -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic
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Research areas
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Orphan Nuclear Receptors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Images
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Chemical Structure - Betulinic acid, TGR5 agonist (ab120654)2D chemical structure image of ab120654, Betulinic acid, TGR5 agonist
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Functional Studies - Betulinic acid, TGR5 agonist (ab120654)ab32557 staining p38 (phospho T180 + Y182) in MALME-3M cells treated with betulinic acid (ab120654), by ICC/IF. Increase in expression of p38 (phospho T180 + Y182) correlates with increased concentration of betulinic acid, as described in literature.
The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120654 (betulinic acid ) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32557 (1/200 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (1)
ab120654 has been referenced in 1 publication.
- Stone SL et al. T-bet Transcription Factor Promotes Antibody-Secreting Cell Differentiation by Limiting the Inflammatory Effects of IFN-? on B Cells. Immunity 50:1172-1187.e7 (2019). PubMed: 31076359