Betulinic acid, TGR5 agonist (ab120654)
- Datasheet
- References
Overview
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Product nameBetulinic acid, TGR5 agonist
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DescriptionAnticancer agent. TGR5 agonist.
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Biological descriptionTriterpenoid with various biological activities. Apoptosis inducer, with anticancer properties. Also shows anti-HIV, antimalarial and anti-inflammatory activity. TGR5 agonist (EC50 = 1.04 μM).
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CAS Number472-15-1
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Chemical structure
Properties
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Chemical name(3β)-3-Hydroxylup-20(29)-en-28-oic acid
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Molecular weight456.70
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Molecular formulaC30H48O3
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PubChem identifier64971
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Storage instructionsStore at +4°C. The product can be stored for up to 12 months.
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Solubility overviewSoluble in DMSO to 50 mM (with warming)
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Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic
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Research areas
Images
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Functional Studies - Betulinic acid, TGR5 agonist (ab120654)ab32557 staining p38 (phospho T180 + Y182) in MALME-3M cells treated with betulinic acid (ab120654), by ICC/IF. Increase in expression of p38 (phospho T180 + Y182) correlates with increased concentration of betulinic acid, as described in literature.
The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120654 (betulinic acid ) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32557 (1/200 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
References
This product has been referenced in:
- Genet C et al. Structure-activity relationship study of betulinic acid, a novel and selective TGR5 agonist, and its synthetic derivatives: potential impact in diabetes. J Med Chem 53:178-90 (2010). Read more (PubMed: 19911773) »
- Fulda S Betulinic Acid for cancer treatment and prevention. Int J Mol Sci 9:1096-107 (2008). Read more (PubMed: 19325847) »
- Yogeeswari P & Sriram D Betulinic acid and its derivatives: a review on their biological properties. Curr Med Chem 12:657-66 (2005). Read more (PubMed: 15790304) »
