Overview

  • Product name
  • Description
    Rabbit polyclonal to BEX3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human BEX3.

  • General notes

    Previously labelled as NADE.

Properties

Applications

Our Abpromise guarantee covers the use of ab21303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 13 kDa).

Target

  • Function
    May be a signaling adapter molecule involved in p75NTR-mediated apoptosis induced by NGF. Plays a role in zinc-triggered neuronal death (By similarity). May play an important role in the pathogenesis of neurogenetic diseases.
  • Tissue specificity
    Found in ovarian granulosa cells, testis, prostate and seminal vesicle tissue. High levels also detected in liver.
  • Sequence similarities
    Belongs to the BEX family.
  • Domain
    The nuclear export signal is required for export from the nucleus and the interactions with itself and p75NTR/NGFR.
  • Post-translational
    modifications
    Ubiquitinated. Degraded by the proteasome.
  • Cellular localization
    Nucleus. Cytoplasm. Shuttles between the cytoplasm and the nucleus. Associates with replicating mitochondria.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bex antibody
    • BEX3 antibody
    • BEX3_HUMAN antibody
    • Brain expressed X linked 3 (mouse) homolog antibody
    • brain expressed, X-linked 3 antibody
    • Brain-expressed X-linked gene 3 antibody
    • Brain-expressed X-linked protein 3 antibody
    • DXS6984E antibody
    • HGR74 antibody
    • NADE antibody
    • Nerve growth factor receptor (TNFRSF16) associated protein 1 antibody
    • Nerve growth factor receptor associated protein 1 antibody
    • Nerve growth factor receptor-associated protein 1 antibody
    • NGFR-associated protein 1 antibody
    • NGFRAP1 antibody
    • Ovarian granulosa cell 13.0 kDa protein HGR74 antibody
    • ovarian granulosa cell protein (13kD) antibody
    • Ovarian granulosa cell protein antibody
    • p75NTR associated cell death executor antibody
    • p75NTR-associated cell death executor antibody
    • Protein BEX3 antibody
    see all

Images

  • All lanes : Anti-BEX3 antibody (ab21303) at 1 µg/ml

    Lane 1 : human brain cell lysate with blocking peptide.
    Lane 2 : human brain cell lysate with Without blocking peptide.

    Predicted band size: 13 kDa
    Observed band size: 23 kDa
    why is the actual band size different from the predicted?

  • ab21303 at 2µg/ml staining BEX3 in human brain tissue by IHC 

References

This product has been referenced in:
  • Xiao L & Tsutsui T Three-dimensional epithelial and mesenchymal cell co-cultures form early tooth epithelium invagination-like structures: Expression patterns of relevant molecules. J Cell Biochem : (2012). Read more (PubMed: 22234822) »
See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Small Cell Lung Cancer)
Gel Running Conditions
Reduced Denaturing (15% Gel)
Loading amount
30 µg
Specification
Small Cell Lung Cancer
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Nov 25 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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