Overview

  • Product name

  • Description

    Rabbit polyclonal to BFL-1/GRS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Chemical/ Small Molecule corresponding to Human BFL-1/GRS.
    (Peptide available as ab53334)

  • General notes

    Previously labelled as Bcl2A1.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, 1.0% BSA, PBS, pH 7.2
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
IP
WB
  • Application notes
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at a concentration of 1-4 µg/ml. Predicted molecular weight: 20 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection.
    • Tissue specificity

      Seems to be restricted to the hematopoietic compartment. Expressed in peripheral blood, spleen, and bone marrow, at moderate levels in lung, small intestine and testis, at a minimal levels in other tissues. Also found in vascular smooth muscle cells and hematopoietic malignancies.
    • Sequence similarities

      Belongs to the Bcl-2 family.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • ACC 1 antibody
      • ACC 2 antibody
      • B2LA1_HUMAN antibody
      • Bcl 2 related protein A1 antibody
      • Bcl-2-like protein 5 antibody
      • Bcl-2-related protein A1 antibody
      • BCL2 related protein A1 antibody
      • Bcl2-L-5 antibody
      • BCL2A1 antibody
      • BCL2L5 antibody
      • BFL1 antibody
      • GRS antibody
      • HBPA1 antibody
      • Hematopoietic BCL2 related protein A1 antibody
      • Hemopoietic specific early response protein antibody
      • Hemopoietic-specific early response protein antibody
      • Protein BFL 1 antibody
      • Protein BFL-1 antibody
      • Protein GRS antibody
      see all

    References

    ab24502 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    I'm sorry to hear the customer is having a problem with ab24502. I have noticed a few parts of the protocol that may explain why the result is a non-specific band and a specific band. For example, the datasheet expresses states to store the antibody at -20 to -80C. It is entirely possible that the antibody is not stable for the past three weeks at 4 degrees. I would also like to suggest the following modifications to your protocol, however, in hopes that the results will improve: 1) Please be consistent with blocking reagent. I would recommend blocking in 5% milk 1 hr, and incubate in primary and secondary with 5% milk. 2) The primary can be diluted further. 3) Can you clarify the sample type? For example, A1 seems to be restricted to the hematopoietic compartment. It is expressed in peripheral blood, spleen, and bone marrow, at moderate levels in lung, small intestine and testis, at a minimal levels in other tissues. It is also found in vascular smooth muscle cells and hematopoietic malignancies. What are WCL? Whole cultured lymphocytes? 4) Can you please send me a labeled picture of the blot as I will be better able to trouble-shoot with this picture? Please let me know if this helps and do not hesitate to contact us for further advice.

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