Overview

  • Product name

  • Description

    Rabbit polyclonal to BFL-1/GRS
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for Bcl2A1 / GRS.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human BFL-1/GRS (C terminal).

  • Positive control

    • Human kidney tissue lysate
  • General notes

    Previously labelled as Bcl2A1.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab45413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 20 kDa.

Target

  • Function

    Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection.
  • Tissue specificity

    Seems to be restricted to the hematopoietic compartment. Expressed in peripheral blood, spleen, and bone marrow, at moderate levels in lung, small intestine and testis, at a minimal levels in other tissues. Also found in vascular smooth muscle cells and hematopoietic malignancies.
  • Sequence similarities

    Belongs to the Bcl-2 family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACC 1 antibody
    • ACC 2 antibody
    • B2LA1_HUMAN antibody
    • Bcl 2 related protein A1 antibody
    • Bcl-2-like protein 5 antibody
    • Bcl-2-related protein A1 antibody
    • BCL2 related protein A1 antibody
    • Bcl2-L-5 antibody
    • BCL2A1 antibody
    • BCL2L5 antibody
    • BFL1 antibody
    • GRS antibody
    • HBPA1 antibody
    • Hematopoietic BCL2 related protein A1 antibody
    • Hemopoietic specific early response protein antibody
    • Hemopoietic-specific early response protein antibody
    • Protein BFL 1 antibody
    • Protein BFL-1 antibody
    • Protein GRS antibody
    see all

Images

  • All lanes : Anti-BFL-1/GRS antibody (ab45413) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate
    Lane 2 : Human lung tissue lysate

    Predicted band size: 20 kDa

  • ab45413 at 10µg/ml staining BFL-1/GRS in mouse kidney tissue by IHC

  • Immunofluorescence of BFL-1/GRS in Mouse Kidney cells using ab45413 at 20 ug/ml.

References

This product has been referenced in:

  • Wu CT  et al. MicroRNA-326 aggravates acute lung injury in septic shock by mediating the NF-?B signaling pathway. Int J Biochem Cell Biol 101:1-11 (2018). IHC-P, WB ; Mouse . Read more (PubMed: 29727715) »
  • Jauharoh SN  et al. SS-A/Ro52 promotes apoptosis by regulating Bcl-2 production. Biochem Biophys Res Commun 417:582-7 (2012). WB ; Human . Read more (PubMed: 22178074) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.

Catalog number ab45413 anti-Bcl2A1 antibody is specific to isoform 1 of Bfl-1 (per Uniprot Q16548).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (HL60)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
HL60
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

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Verified customer

Submitted Jun 20 2007

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