Anti-BFL-1/GRS antibody (ab45413)
Key features and details
- Rabbit polyclonal to BFL-1/GRS
- Suitable for: IHC-P, WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-BFL-1/GRS antibody
See all BFL-1/GRS primary antibodies -
Description
Rabbit polyclonal to BFL-1/GRS -
Host species
Rabbit -
Specificity
This antibody is specific for Bcl2A1 / GRS.
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Tested applications
Suitable for: IHC-P, WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human BFL-1/GRS (C terminal).
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Positive control
- WB: Human lung and kidney tissue. IHC: Mouse kidney tissue. ICC: Mouse kidney cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab45413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 10 - 20 µg/ml.
IHC-P suitable for Mouse samples only |
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 20 kDa.
WB suitable for Human samples only |
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ICC |
Use a concentration of 20 µg/ml.
ICC suitable for Mouse samples only |
Notes |
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IHC-P
Use a concentration of 10 - 20 µg/ml. IHC-P suitable for Mouse samples only |
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 20 kDa. WB suitable for Human samples only |
ICC
Use a concentration of 20 µg/ml. ICC suitable for Mouse samples only |
Target
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Function
Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection. -
Tissue specificity
Seems to be restricted to the hematopoietic compartment. Expressed in peripheral blood, spleen, and bone marrow, at moderate levels in lung, small intestine and testis, at a minimal levels in other tissues. Also found in vascular smooth muscle cells and hematopoietic malignancies. -
Sequence similarities
Belongs to the Bcl-2 family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 597 Human
- Entrez Gene: 12044 Mouse
- Omim: 601056 Human
- SwissProt: Q16548 Human
- SwissProt: Q07440 Mouse
- Unigene: 227817 Human
- Unigene: 425593 Mouse
- Unigene: 479217 Mouse
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Alternative names
- ACC 1 antibody
- ACC 2 antibody
- B2LA1_HUMAN antibody
see all
Images
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All lanes : Anti-BFL-1/GRS antibody (ab45413) at 1 µg/ml
Lane 1 : Human kidney tissue lysate
Lane 2 : Human lung tissue lysate
Predicted band size: 20 kDa -
ab45413 at 10µg/ml staining BFL-1/GRS in mouse kidney tissue by IHC
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Immunocytochemistry of BFL-1/GRS in Mouse Kidney cells using ab45413 at 20 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab45413 has been referenced in 9 publications.
- Kohli J et al. Targeting anti-apoptotic pathways eliminates senescent melanocytes and leads to nevi regression. Nat Commun 13:7923 (2022). PubMed: 36564381
- Marques-Piubelli ML et al. Expression of BCL2 alternative proteins and association with outcome in CLL patients treated with venetoclax. Leuk Lymphoma 62:1129-1135 (2021). PubMed: 33327833
- Hao Y et al. Transcriptomic Signatures and Functional Network Analysis of Chronic Rhinosinusitis With Nasal Polyps. Front Genet 12:609754 (2021). PubMed: 33603773
- He M et al. MicroRNA-664a-3p inhibits the proliferation of ovarian granulosa cells in polycystic ovary syndrome and promotes apoptosis by targeting BCL2A1. Ann Transl Med 9:852 (2021). PubMed: 34164486
- Liang R et al. The Stress-Inducible BCL2A1 Is Required for Ovarian Cancer Metastatic Progression in the Peritoneal Microenvironment. Cancers (Basel) 13:N/A (2021). PubMed: 34572804
- Wu CT et al. MicroRNA-326 aggravates acute lung injury in septic shock by mediating the NF-?B signaling pathway. Int J Biochem Cell Biol 101:1-11 (2018). IHC-P, WB ; Mouse . PubMed: 29727715
- Lu W et al. Fordin: A novel type I ribosome inactivating protein from Vernicia fordii modulates multiple signaling cascades leading to anti-invasive and pro-apoptotic effects in cancer cells in vitro. Int J Oncol 53:1027-1042 (2018). PubMed: 30015835
- Jauharoh SN et al. SS-A/Ro52 promotes apoptosis by regulating Bcl-2 production. Biochem Biophys Res Commun 417:582-7 (2012). WB ; Human . PubMed: 22178074
- Fardin P et al. Induction of epithelial mesenchimal transition and vasculogenesis in the lenses of Dbl oncogene transgenic mice. PLoS One 4:e7058 (2009). IHC-P ; Mouse . PubMed: 19759912