Product nameAnti-BFL-1/GRS antibody
See all BFL-1/GRS primary antibodies
DescriptionRabbit polyclonal to BFL-1/GRS
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Fusion protein corresponding to Human BFL-1/GRS.
Previously labelled as Bcl2A1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Our Abpromise guarantee covers the use of ab75887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
Tested on transfected cells and endogenous protein in various lympoid cell lines.
|IP||Use 1-2µl for 106 cells.
FunctionRetards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection.
Tissue specificitySeems to be restricted to the hematopoietic compartment. Expressed in peripheral blood, spleen, and bone marrow, at moderate levels in lung, small intestine and testis, at a minimal levels in other tissues. Also found in vascular smooth muscle cells and hematopoietic malignancies.
Sequence similaritiesBelongs to the Bcl-2 family.
- Information by UniProt
- ACC 1 antibody
- ACC 2 antibody
- B2LA1_HUMAN antibody
Western blot using ab75887 (1:1000)
Lane 1: Invitro translated human BFL-1/GRS.
Lane 2-5: Immunoprecipitates with:
Lane 2: Ab75887 = anti-GST human BFL-1/GRS (test)
Lane 3: Normal rabbit serum
Lane 4: Anti-HA monoclonal 12CA5
Lane 5: Normal mouse serum
Lane 6: Total lysate
Lanes 7-10: Immunoprecipitates with:
Lane 7: Ab75887
Lane 8: Normal rabbit serum
Lane 9: Anti-FLAG monoclonal M2
Lane 10: Normal mouse serum
Lane 11: Total lysate
Lanes 1-4: Immunoprecipitates with:
Lane 1: Ab75887
Lane 2: Normal rabbit serum
Lane 3: Anti-HA monoclonal 12CA5
Lane 4: Normal rabbit serum
Lane 5: Total lysate
Lanes 6-9: immunoprecipitates with:
Lane 6: Ab75887
Lane 7: Normal rabbit serum
Lane 8: Anti-FLAG monoclonal M2
Lane 9: Normal mouse serum
Lane 10: Total lysate.
Ab75887 was tested on human B cell lines by Dr Natalie Bonnefoy-Berard and colleagues.
Detection of endogenous human BFL-1/GRS in total lysates of B cell lines and in transfected 3T3 and 293T cells by ab75887.
Expression in B cell lines as detected by ab75887 correlates with mRNA levels as detected by RT-PCR, further confirming the specificity of the antibody.
This product has been referenced in:
- Chien WW et al. Differential mechanisms of asparaginase resistance in B-type acute lymphoblastic leukemia and malignant natural killer cell lines. Sci Rep 5:8068 (2015). WB ; Human . Read more (PubMed: 25626693) »
- Olsson Åkefeldt S et al. Chemoresistance of human monocyte-derived dendritic cells is regulated by IL-17A. PLoS One 8:e56865 (2013). WB ; Human . Read more (PubMed: 23441221) »