Anti-BFSP2 antibody (ab122476)
Key features and details
- Rabbit polyclonal to BFSP2
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BFSP2 antibody -
Description
Rabbit polyclonal to BFSP2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: LSRNYEEDVK LLHKQLAGCE LEQMDAPIGT GLDDILETIR IQWERDVEKN RVEAGALLQA KQQAEVAHMS QTQEEKLAAA LRVE, corresponding to internal sequence amino acids 237-320 of Human BFSP2.
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Positive control
- Human lateral ventricle tissue; A 431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.
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Notes |
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IHC-P
1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization. |
Target
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Function
Involved in stabilization of lens fiber cell cytoskeleton. -
Tissue specificity
Lens. -
Involvement in disease
Defects in BFSP2 are the cause of cataract autosomal dominant BFSP2-related (ADC-BFSP2) [MIM:611597]; also known as cataract autosomal dominant multiple types 1. Cataract is an opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. Cataract autosomal dominant BFSP2-related is characterized by a variable phenotype that may or may not be consistent within a family. The opacities can be nuclear, sutural, stellate cortical, lamellar, cortical, nuclear embryonic, Y-sutural, punctate cortical, congenital or with juvenile- and adult-onset. -
Sequence similarities
Belongs to the intermediate filament family. -
Cellular localization
Membrane. Cytoplasm. Cytoplasm > cytoskeleton. Membrane- and cytoskeleton-associated. - Information by UniProt
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Database links
- Entrez Gene: 8419 Human
- Omim: 603212 Human
- SwissProt: Q13515 Human
- Unigene: 659862 Human
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Alternative names
- 49 kDa cytoskeletal protein antibody
- Beaded filament protein CP49 antibody
- Beaded filament structural protein 2 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab122476 has not yet been referenced specifically in any publications.