Overview

  • Product name

    Anti-BHC80 / PHF21A antibody
  • Description

    Rabbit polyclonal to BHC80 / PHF21A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human BHC80/ PHF21A aa 312-487.
    Database link: Q96BD5

  • Positive control

    • WB: Mouse liver and kidney tissue lysate. ICC/IF: HeLa cells. IHC-P: Human liver cancer tissue and adrenal gland tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab224612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
WB 1/500 - 1/5000.
IHC-P 1/20 - 1/200.

Target

  • Function

    Component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it may act as a scaffold. Inhibits KDM1A-mediated demethylation of 'Lys-4' of histone H3 in vitro, suggesting a role in demethylation regulation.
  • Tissue specificity

    Highly expressed in brain. Expressed at much lower level in other tissues.
  • Sequence similarities

    Contains 1 A.T hook DNA-binding domain.
    Contains 1 PHD-type zinc finger.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • BHC80a antibody
    • BM-006 antibody
    • BRAF35-HDAC complex protein BHC80 antibody
    • BRAF35/HDAC2 complex (80 kDa) antibody
    • KIAA1696 antibody
    • PF21A_HUMAN antibody
    • PHD finger protein 21A antibody
    • PHF21A antibody
    see all

Images

  • All lanes : Anti-BHC80 / PHF21A antibody (ab224612) at 1/500 dilution

    Lane 1 : Mouse liver tissue
    Lane 2 : Mouse kidney tissue

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for BHC80 / PHF21A (green) using ab224612 (1/100 dilution) in ICC/IF. Secondary: Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

  • Paraffin embedded human adrenal gland tissue stained for BHC80 / PHF21A with ab224612 (1/100 dilution) in immunohistochemical analysis.

  • Paraffin embedded human liver cancer tissue stained for BHC80 / PHF21A with ab224612 (1/100 dilution) in immunohistochemical analysis.

References

ab224612 has not yet been referenced specifically in any publications.

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