Key features and details
- Rabbit polyclonal to BHC80 / PHF21A
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
Product nameAnti-BHC80 / PHF21A antibody
DescriptionRabbit polyclonal to BHC80 / PHF21A
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human BHC80/ PHF21A aa 312-487.
Database link: Q96BD5
- WB: Mouse liver and kidney tissue lysate. ICC/IF: HeLa cells. IHC-P: Human liver cancer tissue and adrenal gland tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab224612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/5000.|
|IHC-P||1/20 - 1/200.|
FunctionComponent of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it may act as a scaffold. Inhibits KDM1A-mediated demethylation of 'Lys-4' of histone H3 in vitro, suggesting a role in demethylation regulation.
Tissue specificityHighly expressed in brain. Expressed at much lower level in other tissues.
Sequence similaritiesContains 1 A.T hook DNA-binding domain.
Contains 1 PHD-type zinc finger.
- Information by UniProt
- BHC80a antibody
- BM-006 antibody
- BRAF35-HDAC complex protein BHC80 antibody
All lanes : Anti-BHC80 / PHF21A antibody (ab224612) at 1/500 dilution
Lane 1 : Mouse liver tissue
Lane 2 : Mouse kidney tissue
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for BHC80 / PHF21A (green) using ab224612 (1/100 dilution) in ICC/IF. Secondary: Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Paraffin embedded human adrenal gland tissue stained for BHC80 / PHF21A with ab224612 (1/100 dilution) in immunohistochemical analysis.
Paraffin embedded human liver cancer tissue stained for BHC80 / PHF21A with ab224612 (1/100 dilution) in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224612 has not yet been referenced specifically in any publications.