Overview

  • Product name

  • Description

    Rabbit polyclonal to BHLHB5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Hamster
  • Immunogen

    Recombinant fragment corresponding to Human BHLHB5 aa 14-80.
    Sequence:

    EDDLFLHKSLSASTSKRLEAAFRSTPPGMDLSLAPPPRERPASSSSSPLG CFEPADPEGAGLLLPPP


    Database link: Q8NFJ8

  • Positive control

    • Human cerebellum tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab204791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. Despite the presence of an intact basic domain, does not bind to DNA.
  • Tissue specificity

    Brain-specific, with the highest expression in the cerebellum.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Basic helix loop helix domain containing class B 5 1 2 5 antibody
    • Basic helix loop helix protein 5 antibody
    • Beta3 antibody
    • Beta3a antibody
    • BHE22_HUMAN antibody
    • bHLHB5 antibody
    • bHLHe22 antibody
    • CAGL85 antibody
    • Class B basic helix-loop-helix protein 5 antibody
    • Class E basic helix-loop-helix protein 22 antibody
    • Class II bHLH protein BHLHB5 antibody
    • TNRC20 antibody
    • Trinucleotide repeat containing 20 antibody
    • Trinucleotide repeat-containing gene 20 protein antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human cerebellum tissue labeling BHLHB5 with ab204791 at 1/2500 dilution.

References

ab204791 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain, PFA fixed)
Specification
Brain, PFA fixed

Abcam user community

Verified customer

Submitted Jul 26 2018

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Embryonic mid-brain (E12.5 & E14.5))
Specification
Embryonic mid-brain (E12.5 & E14.5)

Neoklis Makrides

Verified customer

Submitted Feb 08 2017

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