Anti-BHLHB5 antibody (ab223261)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-BHLHB5 antibody
See all BHLHB5 primary antibodies -
Description
Goat polyclonal to BHLHB5 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human BHLHB5 aa 128-141 (Cysteine residue).
Sequence:C-KYGESASRGSVAES
Database link: Q8NFJ8 -
Positive control
- WB: Human cerebellum lysate.
Properties
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Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab223261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). |
Target
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Function
Inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. Despite the presence of an intact basic domain, does not bind to DNA. -
Tissue specificity
Brain-specific, with the highest expression in the cerebellum. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 27319 Human
- Omim: 613483 Human
- SwissProt: Q8NFJ8 Human
- Unigene: 388788 Human
- Unigene: 591870 Human
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Alternative names
- Basic helix loop helix domain containing class B 5 1 2 5 antibody
- Basic helix loop helix protein 5 antibody
- Beta3 antibody
see all
Images
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Western blot - Anti-BHLHB5 antibody (ab223261)Anti-BHLHB5 antibody (ab223261) at 1 µg/ml + Human cerebellum lysate at 35 µg
Predicted band size: 37 kDa
Observed band size: 37 kDaPrimary incubation: 1 hour
Protocols
Datasheets and documents
References
ab223261 has not yet been referenced specifically in any publications.
