Overview

  • Product name

  • Description

    Rabbit polyclonal to BHMT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Zebrafish
  • Immunogen

    Recombinant fragment within Zebrafish BHMT aa 2-258. The exact sequence is proprietary.
    Database link: Q32LQ4

  • Positive control

    • Zebrafish whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab211119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 44 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Alternatively Tris-EDTA buffer (pH8.0) may be used.

Target

  • Function

    Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
  • Tissue specificity

    Found exclusively in liver and kidney.
  • Pathway

    Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2.
    Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.
  • Sequence similarities

    Contains 1 Hcy-binding domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

    • Alternative names

      • Betaine homocysteine methyltransferase antibody
      • Betaine homocysteine S methyltransferase 1 antibody
      • Betaine homocysteine S methyltransferase antibody
      • Betaine--homocysteine S-methyltransferase 1 antibody
      • BHMT antibody
      • BHMT1 antibody
      • BHMT1_HUMAN antibody
      • EC 2.1.1.5 antibody
      • Epididymis secretory sperm binding protein Li 61p antibody
      • HEL S 61p antibody
      see all

    Images

    • Paraffin embedded zebrafish tissue stained for BHMT with ab131038 (1/300) in Immunohistochemical analysis.

    • Anti-BHMT antibody (ab211119) at 1/1000 dilution + Zebrafish whole cell lysate at 30 µg

      Predicted band size: 44 kDa



      10% SDS-PAGE

    References

    ab211119 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab211119.
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