Recombinant Anti-BHMT antibody [EP6781] (ab137095)
![Western blot - Anti-BHMT antibody [EP6781] (ab137095)](https://www.abcam.com/ps/products/137/ab137095/Images/ab137095-151689-anti-bhmt-antibody-ep6781-western-blot.jpg "Western blot - Anti-BHMT antibody [EP6781] (ab137095)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP6781] to BHMT
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-BHMT antibody [EP6781]
See all BHMT primary antibodies -
Description
Rabbit monoclonal [EP6781] to BHMT -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human BHMT (N terminal). The exact sequence is proprietary.
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Positive control
- Human fetal liver and fetal kidney lysates; Human kidney and liver tissues.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP6781 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
|
|
| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 45 kDa. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline. -
Tissue specificity
Found exclusively in liver and kidney. -
Pathway
Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2.
Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1. -
Sequence similarities
Contains 1 Hcy-binding domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 635 Human
- Omim: 602888 Human
- SwissProt: Q93088 Human
- Unigene: 80756 Human
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Alternative names
- Betaine homocysteine methyltransferase antibody
- Betaine homocysteine S methyltransferase 1 antibody
- Betaine homocysteine S methyltransferase antibody
see all
Images
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Western blot - Anti-BHMT antibody [EP6781] (ab137095)All lanes : Anti-BHMT antibody [EP6781] (ab137095) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT antibody [EP6781] (ab137095)](https://www.abcam.com/ps/products/137/ab137095/Images/ab137095-151688-anti-bhmt-antibody-ep6781-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT antibody [EP6781] (ab137095)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling BHMT with ab137095 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT antibody [EP6781] (ab137095)](https://www.abcam.com/ps/products/137/ab137095/Images/ab137095-151687-anti-bhmt-antibody-ep6781-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT antibody [EP6781] (ab137095)Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling BHMT with ab137095 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-BHMT antibody [EP6781] (ab137095)](https://www.abcam.com/ps/products/137/ab137095/Images/ab137095-4-benefits-of-recombinant-antibodies.png)
Anti-BHMT antibody [EP6781] (ab137095)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab137095 has not yet been referenced specifically in any publications.