Recombinant
RabMAb

Recombinant Anti-BHMT antibody [EP6781] (ab137095)

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Overview

  • Product name

    Anti-BHMT antibody [EP6781]
    See all BHMT primary antibodies

  • Description

    Rabbit monoclonal [EP6781] to BHMT

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human BHMT (N terminal). The exact sequence is proprietary.

  • Positive control

    • Human fetal liver and fetal kidney lysates; Human kidney and liver tissues.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC or IP.

    • Target

    • Function

      Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.

    • Tissue specificity

      Found exclusively in liver and kidney.

    • Pathway

      Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2.
      Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.

    • Sequence similarities

      Contains 1 Hcy-binding domain.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      • Alternative names

        • Betaine homocysteine methyltransferase antibody
        • Betaine homocysteine S methyltransferase 1 antibody
        • Betaine homocysteine S methyltransferase antibody
        • Betaine--homocysteine S-methyltransferase 1 antibody
        • BHMT antibody
        • BHMT1 antibody
        • BHMT1_HUMAN antibody
        • EC 2.1.1.5 antibody
        • Epididymis secretory sperm binding protein Li 61p antibody
        • HEL S 61p antibody
        see all

      Images

      • Western blot - Anti-BHMT antibody [EP6781] (ab137095)
        Western blot - Anti-BHMT antibody [EP6781] (ab137095)
        All lanes : Anti-BHMT antibody [EP6781] (ab137095) at 1/1000 dilution

        Lane 1 : Human fetal liver lysate
        Lane 2 : Human fetal kidney lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 45 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT antibody [EP6781] (ab137095)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT antibody [EP6781] (ab137095)

        Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling BHMT with ab137095 at 1/250 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT antibody [EP6781] (ab137095)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT antibody [EP6781] (ab137095)

        Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling BHMT with ab137095 at 1/250 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      References

      ab137095 has not yet been referenced specifically in any publications.

      Publishing research using ab137095? Please let us know so that we can cite the reference in this datasheet.

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