Overview

  • Product name

    Anti-BHMT antibody [EP6781]
    See all BHMT primary antibodies
  • Description

    Rabbit monoclonal [EP6781] to BHMT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human BHMT (N terminal). The exact sequence is proprietary.

  • Positive control

    • Human fetal liver and fetal kidney lysates; Human kidney and liver tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function

      Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
    • Tissue specificity

      Found exclusively in liver and kidney.
    • Pathway

      Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2.
      Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.
    • Sequence similarities

      Contains 1 Hcy-binding domain.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • Betaine homocysteine methyltransferase antibody
      • Betaine homocysteine S methyltransferase 1 antibody
      • Betaine homocysteine S methyltransferase antibody
      • Betaine--homocysteine S-methyltransferase 1 antibody
      • BHMT antibody
      • BHMT1 antibody
      • BHMT1_HUMAN antibody
      • EC 2.1.1.5 antibody
      • Epididymis secretory sperm binding protein Li 61p antibody
      • HEL S 61p antibody
      see all

    Images

    • All lanes : Anti-BHMT antibody [EP6781] (ab137095) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Human fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 45 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling BHMT with ab137095 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling BHMT with ab137095 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab137095 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab137095.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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