Overview

  • Product name

    Anti-BHMT antibody [EPR20076]
    See all BHMT primary antibodies
  • Description

    Rabbit monoclonal [EPR20076] to BHMT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human BHMT aa 1-350. The exact sequence is proprietary.
    Database link: Q93088

  • Positive control

    • WB: Human BHMT1 recombinant protein; Human fetal kidney and fetal liver lysates; Mouse and rat kidney and liver lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab207765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

Target

  • Function

    Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
  • Tissue specificity

    Found exclusively in liver and kidney.
  • Pathway

    Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2.
    Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.
  • Sequence similarities

    Contains 1 Hcy-binding domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Betaine homocysteine methyltransferase antibody
    • Betaine homocysteine S methyltransferase 1 antibody
    • Betaine homocysteine S methyltransferase antibody
    • Betaine--homocysteine S-methyltransferase 1 antibody
    • BHMT antibody
    • BHMT1 antibody
    • BHMT1_HUMAN antibody
    • EC 2.1.1.5 antibody
    • Epididymis secretory sperm binding protein Li 61p antibody
    • HEL S 61p antibody
    see all

Images

  • All lanes : Anti-BHMT antibody [EPR20076] (ab207765) at 1/50000 dilution

    Lane 1 : Human BHMT1 recombinant protein
    Lane 2 : Human BHMT2 recombinant protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human BHMT1 and BHMT2 recombinant proteins contain aa11-314 and aa1-363 respectively, and both are with a His-tag.

  • All lanes : Anti-BHMT antibody [EPR20076] (ab207765) at 1/10000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human brain lysate
    Lane 3 : Human lung lysate
    Lane 4 : Human spleen lysate
    Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 1 second


    5% NFDM/TBST: Blocking and dilution buffer.

    BHMT1 is mainly expressed in liver and kidney according to the literature (PMID: 9281325). Negative control: HepG2 (PMID: 22138536).

  • Anti-BHMT antibody [EPR20076] (ab207765) at 1/5000 dilution + Human fetal liver lysate at 1 µg

    Secondary
    Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Lane 1 : Anti-BHMT antibody [EPR20076] (ab207765) at 1/1000 dilution
    Lane 2 : Anti-BHMT antibody [EPR20076] (ab207765) at 1/5000 dilution
    Lanes 3-4 : Anti-BHMT antibody [EPR20076] (ab207765) at 1/20000 dilution

    Lane 1 : Rat kidney lysate at 10 µg
    Lane 2 : Mouse kidney lysate at 10 µg
    Lane 3 : Mouse liver lysate at 0.5 µg
    Lane 4 : Rat liver lysate at 0.5 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/3/4: 1 second; Lane 2: 3 minutes.

References

ab207765 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab207765.
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