Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6782] to BHMT - BSA and Azide free
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Product nameAnti-BHMT antibody [EPR6782] - BSA and Azide free
See all BHMT primary antibodies
DescriptionRabbit monoclonal [EPR6782] to BHMT - BSA and Azide free
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human BHMT aa 350-450 (C terminal). The exact sequence is proprietary.
ab248086 is the carrier-free version of ab124992 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248086 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Dissociation constant (KD)KD = 2.25 x 10 -11 M Learn more about KD
Storage bufferpH: 7.2
Concentration information loading...
Our Abpromise guarantee covers the use of ab248086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.
|IP||Use at an assay dependent concentration.|
FunctionInvolved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
Tissue specificityFound exclusively in liver and kidney.
PathwayAmine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2.
Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.
Sequence similaritiesContains 1 Hcy-binding domain.
- Information by UniProt
- Betaine homocysteine methyltransferase antibody
- Betaine homocysteine S methyltransferase 1 antibody
- Betaine homocysteine S methyltransferase antibody
All lanes : Anti-BHMT antibody [EPR6782] (ab124992) at 1/1000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 45 kDa
This data was developed using ab124992, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab248086 has not yet been referenced specifically in any publications.