Overview

  • Product name

  • Description

    Rabbit polyclonal to BHMT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Pig
  • Immunogen

    Recombinant fragment corresponding to Human BHMT2 aa 94-363.
    Database link: Q9H2M3

  • Positive control

    • HCT116 and Mouse liver whole cell lysates; HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab153990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/20000. Predicted molecular weight: 40 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance

    Homocysteine is a sulfur-containing amino acid that plays a crucial role in methylation reactions. Transfer of the methyl group from betaine to homocysteine creates methionine, which donates the methyl group to methylate DNA, proteins, lipids, and other intracellular metabolites. BHMT2 is one of two methyl transferases that can catalyze the transfer of the methyl group from betaine to homocysteine. Anomalies in homocysteine metabolism have been implicated in disorders ranging from vascular disease to neural tube birth defects such as spina bifida.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • Betaine homocysteine methyltransferase 2 antibody
    • Betaine homocysteine S methyltransferase 2 antibody
    • EC 2.1.1.5 antibody
    • FLJ20001 antibody
    • S methylmethionine homocysteine S methyltransferase BHMT2 antibody
    • SMM hcy methyltransferase antibody
    see all

Images

  • Anti-BHMT2 antibody (ab153990) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size: 40 kDa



    10% SDS PAGE
  • Anti-BHMT2 antibody (ab153990) at 1/20000 dilution + Mouse liver whole cell lysate at 20 µg

    Predicted band size: 40 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling BHMT2 with ab153990 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

References

ab153990 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab153990.
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