Key features and details
- Rabbit polyclonal to BHMT2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-BHMT2 antibody
See all BHMT2 primary antibodies
DescriptionRabbit polyclonal to BHMT2
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Orangutan
Recombinant fragment corresponding to Human BHMT2 aa 1-200.
Database link: Q9H2M3
- WB: Mouse liver lysate; Raji whole cell lysate. IHC-P: Human liver tissue. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurtiy >95%.
Our Abpromise guarantee covers the use of ab236947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 40 kDa.|
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/20 - 1/200.|
RelevanceHomocysteine is a sulfur-containing amino acid that plays a crucial role in methylation reactions. Transfer of the methyl group from betaine to homocysteine creates methionine, which donates the methyl group to methylate DNA, proteins, lipids, and other intracellular metabolites. BHMT2 is one of two methyl transferases that can catalyze the transfer of the methyl group from betaine to homocysteine. Anomalies in homocysteine metabolism have been implicated in disorders ranging from vascular disease to neural tube birth defects such as spina bifida.
- Betaine homocysteine methyltransferase 2 antibody
- Betaine homocysteine S methyltransferase 2 antibody
- EC 18.104.22.168 antibody
All lanes : Anti-BHMT2 antibody (ab236947) at 1/1000 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 40 kDa
Paraffin-embedded human liver tissue stained for BHMT2 using ab236947 at 1/100 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for BHMT2 (green) using ab236947 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236947 has not yet been referenced specifically in any publications.