Overview

  • Product name
    Anti-BI-1 antibody
  • Description
    Rabbit polyclonal to BI-1
  • Host species
    Rabbit
  • Specificity
    ab18852 recognises Bax inhibitor.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human BI-1.

  • Positive control
    • rat testis tissue lysate
  • General notes

    Previously labelled as Bax inhibitor 1.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 26 kDa.

Target

Images

  • Anti-BI-1 antibody (ab18852) at 1 µg/ml + rat testis tissue lysate

    Predicted band size: 26 kDa
    Observed band size: 22 kDa
    why is the actual band size different from the predicted?

  • ICC/IF image of ab18852 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18852, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab18852 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your reply.
I am sorry the antibody did not give the expected results following the suggestions.
I am happy to send a free of charge replacement as it is still covered by the Abpromise guarantee. Unfortunately, the only lot available in stock is the same the customer received already. I could therefore send the customer the same lot or an alternative product instead, targeting Bax inhibitor too:
https://www.abcam.com/bax-inhibitor-1-antibody-20f565-ab150615.html
As the previous product, this is recommended for WB in Mouse lysates, so it is also covered by the guarantee in such conditions.
Please let me know whether the customer would like to take this offer up, or a refund or credit note is preferred instead.
I’d also like to bring your attention to an offer we are running throughout November. If you order any primary antibody you can receive a RabMab free, whilst stocks last (we can also apply this offer for free of charge replacement orders). Simply quote “RABMAB-XBSMG” in your next primary antibody order. More information on this offer can be found from the following link:
https://www.abcam.com/index.html?pageconfig=resource&rid=15447
Please let me know how you would like to proceed. I look forward to receiving your reply.

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Question
Answer

I am sorry to hear you have been experiencing problems with one of our antibodies. The quality of our products is important to us and I would like to reassure you that we investigate all customer complaints. I appreciate the time taken to submit this information to us and I would like to make some suggestions to the protocol in an attempt to improve the results obtained.
- Use fresh buffers, daily prepared ones.
- We recommend using reducing conditions unless otherwise stated on the datasheet. This will ensure the protein is in the correct conformation to run at the correct Molecular Weight and for the antibody to run. In order to do it, along with the sample heating for 5-10 minutes, a reducing agent (BME or DTT) is recommended.
- Optimise the blocking step, using 5% milk for 2 hours at RT. Otherwise, 5%BSA may also give better results.
-Run a no primary control to dismiss secondary antibody failure.
-Avoid diluting the antibodies in blocking agent. Instead use TBST, or 1% BSA to do it. Keep overnight incubation but please note it should be done at 4C.
I hope these tips help, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again and I’ll be more than happy to help you further.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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