Overview

  • Product name

    Anti-BICD2 antibody - C-terminal
    See all BICD2 primary antibodies
  • Description

    Rabbit polyclonal to BICD2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human BICD2 (C terminal). The exact sequence is proprietary.
    Database link: Q8TD16

  • Positive control

    • WB: HEK-293T, Jurkat, Raji, NCI-H929, PC-12 and Rat2 whole cell extracts. IHC-P: Mouse testis tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 94 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    May play a role in the dynein-dynactin interactions on the surface of membranous organelles, by associating with these complexes. Regulates coat complex coatomer protein I (COPI)-independent Golgi-endoplasmic reticulum transport by recruiting the dynein-dynactin motor complex.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the BicD family.
  • Domain

    The fourth coiled coil region is involved in Golgi targeting and in the interaction with DCTN2.
  • Post-translational
    modifications

    Phosphorylated by NEK9 in vitro.
  • Cellular localization

    Golgi apparatus. Cytoplasm > cytoskeleton. In interphase cells mainly localizes to the Golgi complex and colocalizes with dynactin at microtubule plus ends (By similarity). Its localization is dependent on microtubule morphology.
  • Information by UniProt
  • Database links

  • Alternative names

    • bA526D8.1 antibody
    • Bic D 2 antibody
    • Bic-D 2 antibody
    • Bicaudal D homolog 2 (Drosophila) antibody
    • Bicaudal D homolog 2 antibody
    • Bicd2 antibody
    • BICD2_HUMAN antibody
    • Coiled coil protein BICD2 antibody
    • Cytoskeleton like bicaudal D protein homolog 2 antibody
    • Homolog of Drosophila bicaudal D antibody
    • KIAA0699 antibody
    • Protein bicaudal D homolog 2 antibody
    see all

Images

  • All lanes : Anti-BICD2 antibody - C-terminal (ab229230) at 1/1000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 94 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded mouse testis tissue stained for BICD2 using ab229230 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-BICD2 antibody - C-terminal (ab229230) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
    Lane 3 : NCI-H929 whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 94 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-BICD2 antibody - C-terminal (ab229230) at 1/1000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with BICD2 shRNA, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 94 kDa



    7.5% SDS-PAGE gel.

References

ab229230 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229230.
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